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Alternative Products
Cat. No. | Product Name | Format | Quantity | Price |
---|---|---|---|---|
65160S |
anti-Plakophilin 1 mouse monoclonal, PP1-5C2 |
supernatant, sample (ready-to-use) | 600 µL (ready-to-use) |
$65.00
Excl. VAT, excl. Shipping Cost
|
70030 |
anti-Desmosome sample set 1 |
600 µl each antibody |
$230.00
|
|
70035 |
anti-Plakophilin 1-3 sample set |
600 µl each antibody |
$139.00
|
Product description
Host | mouse |
---|---|
Antibody Type | monoclonal |
Isotype | IgG1 |
Clone | PP1-5C2 |
Immunogen | plakophilin 1 ("band 6 polypeptide" of bovine muzzle epidermis) |
Purification | hybridoma cell culture supernatant |
Conjugate | unconjugated |
Formulation | contains 0.09% sodium azide |
Storage | short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles |
No. of experiments | ≤ 5000 (IHC, 50 µl/slide) |
Intended use | Research use only |
Tested species reactivity | bovine, human, mouse, pig, rat |
Applications
Tested applications | Tested dilutions |
---|---|
ELISA | assay dependent |
Immunohistochemistry (IHC) - frozen | undiluted - 1:50 (preincubation with 0.05 - 0.2% Triton X-100, for 5-10 min recommended; for delicate tissues like liver and gastrointestinal material preincubation with saponin is preferred) |
Immunohistochemistry (IHC) - paraffin | undiluted - 1:50 (pretreatment with 0.001% trypsin and microwave treatment recommended) |
Western Blot (WB) | assay dependent |
Background
Details
Antibody recognizes plakophilin 1 in diverse stratified epithelia, in complex epithelia (e.g. urothelium of the bladder and tracheal epithelium) and in glandular duct epithelia; squamous cell carcinoma are positive, whereas all adenocarcinoma tested so far are negative.
Polypeptide reacting: Plakophilin 1: calculated mol. wt. 80,496 (human) and 80,180 (bovine).
Reactivity on human cultured cell lines: HaCaT, A-431, BPH-1 (derived from non-neoplastic prostatic tissue)
Reference
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Species
Application
human
IHC (paraffin)
human
IHC (frozen)
ICC-IF
ICC-IF