IHC of mouse tongue (courtesy of J.Heß, University Hospital Heidelberg)

anti-Plakophilin 1 mouse monoclonal, PP1-5C2, supernatant

The anti-plakophilin 1 monoclonal antibody recognizes plakophilin 1 in stratified epithelia. Positive reaction with squamous cell carcinoma, negative with adenocarcinoma.

Cat. No.: 65160
Quantity: 5 mL
No. of experiments: ≤ 5000 (IHC, 50 µl/slide)

Delivery Time: usually 1-7 working days

$245.00

Excl. VAT, excl. Shipping Cost

Qty:

Publication

Species

Application

Domke, L and Franke, W. The cell-cell junctions of mammalian testes.., Cell Tissue Res, 375, 451-482, (2019)

pig

ICC-IF

Yang, C. et al. Plakophilin 1-deficient cells upregulate SPOCK1: implications for prostate cancer progression. Tumour Biol. 36, 9567â77 (2015).

human

IHC (paraffin)

Rickelt, S., Winter-Simanowski, S., Noffz, E., Kuhn, C. & Franke, W. W. Upregulation of plakophilin-2 and its acquisition to adherens junctions identifies a novel molecular ensemble of cell-cell-attachment characteristic for transformed mesenchymal cells.

human

South, A. P. et al. Lack of plakophilin 1 increases keratinocyte migration and reduces desmosome stability. J. Cell Sci. 116, 3303â3314 (2003).

human

ICC-IF

Vasioukhin, V., Bowers, E., Bauer, C., Degenstein, L. & Fuchs, E. Desmoplakin is essential in epidermal sheet formation. Nat. Cell Biol. 3, 1076â1085 (2001).

human

WB
ICC-IF

Hofmann, I. et al. Interaction of plakophilins with desmoplakin and intermediate filament proteins: an in vitro analysis. J. Cell Sci. 113, 2471â83 (2000).

human

IP
IEM

Mertens, C., Kulm, C. & Franke, W. W. Plakophilins 2a and 2b: Constitutive Proteins of Dual Location in the Karyoplasm and the Desmosomal Plaque. J. Cell Biol. 135, 1009â1025 (1996).

human

IHC (frozen)
ICC-IF

Heid, H. W. et al. Cell typespecific desmosomal plaque proteins of the plakoglobin family: plakophilin 1 (band 6 protein). Differentiation 58, 113â131 (1995).

human
bovine
xenopus

WB
IHC (frozen)

Alternative Products

Cat. No.	Product Name	Format	Quantity	Price
65160S	anti-Plakophilin 1 mouse monoclonal, PP1-5C2	supernatant, sample (ready-to-use)	600 µL (ready-to-use)	$65.00 Excl. VAT, excl. Shipping Cost
70030	anti-Desmosome sample set 1		600 µl each antibody	$230.00
70035	anti-Plakophilin 1-3 sample set		600 µl each antibody	$139.00

Product description

Host	mouse
Antibody Type	monoclonal
Isotype	IgG1
Clone	PP1-5C2
Immunogen	plakophilin 1 ("band 6 polypeptide" of bovine muzzle epidermis)
Purification	hybridoma cell culture supernatant
Conjugate	unconjugated
Formulation	contains 0.09% sodium azide
Storage	short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles
No. of experiments	≤ 5000 (IHC, 50 µl/slide)
Intended use	Research use only
Tested species reactivity	bovine, human, mouse, pig, rat

Applications

Tested applications	Tested dilutions
ELISA	assay dependent
Immunohistochemistry (IHC) - frozen	undiluted - 1:50 (preincubation with 0.05 - 0.2% Triton X-100, for 5-10 min recommended; for delicate tissues like liver and gastrointestinal material preincubation with saponin is preferred)
Immunohistochemistry (IHC) - paraffin	undiluted - 1:50 (pretreatment with 0.001% trypsin and microwave treatment recommended)
Western Blot (WB)	assay dependent

Background

Details

Antibody recognizes plakophilin 1 in diverse stratified epithelia, in complex epithelia (e.g. urothelium of the bladder and tracheal epithelium) and in glandular duct epithelia; squamous cell carcinoma are positive, whereas all adenocarcinoma tested so far are negative.

Polypeptide reacting: Plakophilin 1: calculated mol. wt. 80,496 (human) and 80,180 (bovine).

Reactivity on human cultured cell lines: HaCaT, A-431, BPH-1 (derived from non-neoplastic prostatic tissue)

Reference

Publication

Species

Application

Domke, L and Franke, W. The cell-cell junctions of mammalian testes.., Cell Tissue Res, 375, 451-482, (2019)

pig

ICC-IF

Yang, C. et al. Plakophilin 1-deficient cells upregulate SPOCK1: implications for prostate cancer progression. Tumour Biol. 36, 9567â77 (2015).

human

IHC (paraffin)

human

South, A. P. et al. Lack of plakophilin 1 increases keratinocyte migration and reduces desmosome stability. J. Cell Sci. 116, 3303â3314 (2003).

human

ICC-IF

Vasioukhin, V., Bowers, E., Bauer, C., Degenstein, L. & Fuchs, E. Desmoplakin is essential in epidermal sheet formation. Nat. Cell Biol. 3, 1076â1085 (2001).

human

WB
ICC-IF

Hofmann, I. et al. Interaction of plakophilins with desmoplakin and intermediate filament proteins: an in vitro analysis. J. Cell Sci. 113, 2471â83 (2000).

human

IP
IEM

Mertens, C., Kulm, C. & Franke, W. W. Plakophilins 2a and 2b: Constitutive Proteins of Dual Location in the Karyoplasm and the Desmosomal Plaque. J. Cell Biol. 135, 1009â1025 (1996).

human

IHC (frozen)
ICC-IF

Heid, H. W. et al. Cell typespecific desmosomal plaque proteins of the plakoglobin family: plakophilin 1 (band 6 protein). Differentiation 58, 113â131 (1995).

human
bovine
xenopus

WB
IHC (frozen)

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anti-Plakophilin 1 mouse monoclonal, PP1-5C2, supernatant

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