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IHC of mouse tongue (courtesy of J.Heß, University Hospital Heidelberg) Zoom

IHC of mouse tongue (courtesy of J.Heß, University Hospital Heidelberg)

anti-Plakophilin 1 mouse monoclonal, PP1-5C2, supernatant

The anti-plakophilin 1 monoclonal antibody recognizes plakophilin 1 in stratified epithelia. Positive reaction with squamous cell carcinoma, negative with adenocarcinoma.
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Cat. No.: 65160
Quantity:  5 mL
No. of experiments:  ≤ 5000 (IHC, 50 µl/slide)

Delivery Time: usually 1-7 working days

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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone PP1-5C2
Immunogen plakophilin 1 ("band 6 polypeptide" of bovine muzzle epidermis)
Purification hybridoma cell culture supernatant
Conjugate unconjugated
Formulation contains 0.09% sodium azide
Storage short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles
No. of experiments ≤ 5000 (IHC, 50 µl/slide)
Intended use Research use only
Tested species reactivity bovine, human, mouse, pig, rat


Tested applications Tested dilutions
ELISA assay dependent
Immunohistochemistry (IHC) - frozen undiluted - 1:50 (preincubation with 0.05 - 0.2% Triton X-100, for 5-10 min recommended; for delicate tissues like liver and gastrointestinal material preincubation with saponin is preferred)
Immunohistochemistry (IHC) - paraffin undiluted - 1:50 (pretreatment with 0.001% trypsin and microwave treatment recommended)
Western Blot (WB) assay dependent



Antibody recognizes plakophilin 1 in diverse stratified epithelia, in complex epithelia (e.g. urothelium of the bladder and tracheal epithelium) and in glandular duct epithelia; squamous cell carcinoma are positive, whereas all adenocarcinoma tested so far are negative.

Polypeptide reacting: Plakophilin 1: calculated mol. wt. 80,496 (human) and 80,180 (bovine).

Reactivity on human cultured cell lines: HaCaT, A-431, BPH-1 (derived from non-neoplastic prostatic tissue)


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