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rat primary cardiomycytes (courtesy of L. Langbein) Zoom

rat primary cardiomycytes (courtesy of L. Langbein)

anti-Plakophilin 2 guinea pig polyclonal, serum

Plakophilin 2 is a desmosomal plaque protein in simple and glandular epithelia, basal layer of stratified epithelia and in some nonepithelial tissues, e.g. myocard; in some cells devoid of desmosomes, PP2 is enriched in the karyoplasm.
Cat. No.: GP-PP2
Quantity:  100 µl

Delivery Time: usually 1-7 working days

€260.00
Excl. 19% Tax, excl. Shipping Cost

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Product description

Host guinea pig
Antibody Type polyclonal
Immunogen human synthetic plakophilin 2 (C-terminal aa 820-8 37)
Purification stabilized antiserum
Conjugate unconjugated
Formulation contains 0.09% sodium azide and 0.5% BSA
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Tested species reactivity bovine, human, mouse, pig, rat (negative with amphibia and fish)

Applications

Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) assay dependent
Immunohistochemistry (IHC) - frozen 1:100 (preincubated for 5 min with 0.2% Triton X-100 prior to antibody application recommended; longer incubation could result in a loss of nuclear PP2-staining; fixation with 2% formaldehyde to prevent wash-out of soluble forms)
Immunohistochemistry (IHC) - paraffin 1:100 (microwave treatment recommended)
Western Blot (WB) assay dependent

Background

Details

PP2 antiserum stains specifically desmosomal plaques present in simple and glandular epithelia, the basal layer of certain stratified epithelia, all layers of some stratified epithelia, and desmosome-possessing nonepithelial cells such as myocardium, Purkinje fibers, and dendritic reticulum of lymph node follicles. In all these cells, including a variety of cell types devoid of desmosomes, plakophilin 2 is highly enriched in the karyoplasm.
Polypeptide reacting: Plakophilin 2, Mr ~100 kD (SDS-PAGE); MW calculated from aa sequence: 92,750 (pI 9.33) and 97,410 (pI 9.38). 

Reactivity on cultured cell lines: MCF-7, PLC.

Reference

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