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anti-Plakophilin 2 mouse monoclonal, Pkp2-518, supernatant

Plakophilin 2 is a desmosomal plaque protein in simple and glandular epithelia, basal layer of stratified epithelia and in some nonepithelial tissues, e.g. myocard; in some cells devoid of desmosomes, PP2 is enriched in the karyoplasm.
Cat. No.: 651167
Quantity:  5 mL

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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone Pkp2-518
Immunogen Synthetic carboxyterminal peptide (aa 527 - 872) of human plakophilin 2
Purification hybridoma cell culture supernatant
Conjugate unconjugated
Formulation contains 0.09% sodium azide
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Tested species reactivity bovine, human, mouse, ovine, rat (negative with amphibia and fish)

Applications

Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen ready-to-use
Immunohistochemistry (IHC) - paraffin ready-to-use (microwave treatment recommended)
Immuno Electron Microscopy (IEM) assay dependent
Immunoprecipitation (IP) assay dependent
Western Blot (WB) assay dependent

Background

Details

The mab recognizes plakophilin 2 in both locations: in the desmosomal plaque and in the karyoplasm. Plakophilin 2 is a member of the arm-repeat family of proteins which can occur in at least 2 splice forms (2a and 2b). Plakophilin 2 assembles with other proteins to form the desmosomal plaque in simple and glandular epithelia, the basal layer of certain stratified epithelia, all layers of some stratified epithelia, in Merkel cells, and desmosome-possessing nonepithelial cells such as myocardium, Purkinje fibers, and dendritic reticulum of lymph node follicles. In all these cells, including a variety of cell types devoid of desmosomes, plakophilin 2 is highly enriched in the nuclei (karyoplasm).
Polypeptide reacting: Plakophilin 2, Mr ~100 kD (SDS-PAGE); MW calculated from aa sequence: 92,750 (pI 9.33) and 97,410 (pI 9.38)
Positive staining was obtained with several carcinomas derived from simple-type epithelia (e.g. hepatomas, colon carcinomas) and some adenocarcinomas derived from stratified epithelia (oesophagus carcinoma).
Recommendation for immunohistochemistry on frozen sections: For better accessibility of the desmosome-associated antigen frozen sections might be pre-incubated for 5 min in PBS plus 0.2% Triton X-100 prior to antibody application. Longer incubation could result in a loss of Pkp2-nuclear staining. To prevent wash-out of soluble forms of the antigen, fixation with 2% formaldehyde is recommended.

Reference

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