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anti-Plakophilin 2 mouse monoclonal, PP2/62 + PP2/86 + PP2/150, supernatant

Plakophilin 2 is a desmosomal plaque protein in simple and glandular epithelia, basal layer of stratified epithelia and in some nonepithelial tissues, e.g. myocard; in some cells devoid of desmosomes, PP2 is enriched in the karyoplasm.
Cat. No.: 651101
Quantity:  5 mL

Delivery Time: usually 1-7 working days

€196.00
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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone PP2/62 + PP2/86 + PP2/150
Immunogen synthetic carboxyterminal peptide (24 aa) of human plakophilin 2
Purification hybridoma cell culture supernatant
Conjugate unconjugated
Formulation contains 0.09% sodium azide
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
UniprotID Q99959
Tested species reactivity bovine, canine, human, mouse, rat, Xenopus laevis (rat and mouse: Plakophilin 2a and b (WB only))

Applications

Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen ready-to-use (incubate for 5 min in PBS plus 0.2% Triton-X100 prior to antibody application; longer incubation could result in a loss of nuclear staining; fixation with 2% formaldehyde is recommended; exclusive nuckear staining without detergent)
Immunohistochemistry (IHC) - paraffin ready-to-use (microwave treatment recommended)
Immunoprecipitation (IP) assay dependent
Western Blot (WB) assay dependent

Background

Details

The mabs react with plakophilin 2, a member of the arm-repeat family of proteins which can occur in at least 2 splice forms (2a and 2b). Plakophilin 2 assembles with other proteins to form the desmosomal plaque in simple and glandular epithelia, the basal layer of certain stratified epithelia, all layers of some stratified epithelia, and in nonepithelial desmosome-possessing tissues such as myocardium, Purkinje fibers, and dendritic reticulum of lymph node follicles. In all these cells, including a variety of cell types devoid of desmosomes, plakophilin 2 is highly enriched in the karyoplasm. Polypeptide reacting: Plakophilin 2, Mr 100 kD (SDS-PAGE); MW calculated from aa sequence: 92,750 (pI 9.33) and 97,410 (pI 9.38)

Tumors specifically detected: Positive staining was obtained with several carcinomas derived from simple-type epithelia (e.g. hepatomas, colon carcinomas) and some adenocarcinomas derived from stratified epithelia (oesophagus carcinoma). The antibody was negative with all leiomyosarcomas tested.

Reference

Reference

PublicationSpeciesApplication
Dubash, A. D. et al. Plakophilin-2 loss promotes TGF-β1/p38 MAPK-dependent fibrotic gene expression in cardiomyocytes. J. Cell Biol. 212, 425–38 (2016). rat WB, ICC-IF
Kant, S., Holthöfer, B., Magin, T. M., Krusche, C. A. & Leube, R. E. Desmoglein 2-Dependent Arrhythmogenic Cardiomyopathy Is Caused by a Loss of Adhesive Function. Circ. Cardiovasc. Genet. 8, 553–63 (2015). mouse IHC (paraffin)
Rappe, U., Schlechter, T., Aschoff, M., Hotz-Wagenblatt, A. & Hofmann, I. Nuclear ARVCF Protein Binds Splicing Factors and Contributes to the Regulation of Alternative Splicing. J. Biol. Chem. 289, 12421–12434 (2014). human WB
Patel, D. M., Dubash, A. D., Kreitzer, G. & Green, K. J. Disease mutations in desmoplakin inhibit Cx43 membrane targeting mediated by desmoplakin-EB1 interactions. J. Cell Biol. 206, 779–97 (2014). human ICC-IF
Fischer-Kešo, R. et al. Plakophilins 1 and 3 Bind to FXR1 and Thereby Influence the mRNA Stability of Desmosomal Proteins. Mol. Cell. Biol. 34, 4244–4256 (2014). human WB, IP
Rickelt, S. Plakophilin-2: a cell-cell adhesion plaque molecule of selective and fundamental importance in cardiac functions and tumor cell growth. Cell Tissue Res 348, 281–294 (2012). human, mouse WB, IHC (paraffin)
Pieperhoff, S. et al. The plaque protein myozap identified as a novel major component of adhering junctions in endothelia of the blood and the lymph vascular systems. J. Cell. Mol. Med 16, 1709–1719 (2012). mouse WB
Fabritz, L. et al. Load-reducing therapy prevents development of arrhythmogenic right ventricular cardiomyopathy in plakoglobin-deficient mice. J. Am. Coll. Cardiol. 57, 740–750 (2011). mouse IHC
Rickelt, S., Winter-Simanowski, S., Noffz, E., Kuhn, C. & Franke, W. W. Upregulation of plakophilin-2 and its acquisition to adherens junctions identifies a novel molecular ensemble of cell-cell-attachment characteristic for transformed mesenchymal cells. Int. J. cancer 125, 2036–48 (2009). human WB, ICC-IF
Goossens, S. et al. A unique and specific interaction between  T-catenin and plakophilin-2 in the area composita, the mixed-type junctional structure of cardiac intercalated discs. J. Cell Sci. 120, 2126–2136 (2007). mouse WB, IHC (paraffin), IEM
Pieperhoff, S. & Franke, W. W. The area composita of adhering junctions connecting heart muscle cells of vertebrates – IV: Coalescence and amalgamation of desmosomal and adhaerens junction components – Late processes in mammalian heart development. Eur. J. Cell Biol. 86, 377–391 (2007). mouse IHC (frozen)
Koeser, J., Troyanovsky, S. M., Grund, C. & Franke, W. W. De novo formation of desmosomes in cultured cells upon transfection of genes encoding specific desmosomal components. Exp. Cell Res. 285, 114–30 (2003). human WB, ICC-IF
South, A. P. et al. Lack of plakophilin 1 increases keratinocyte migration and reduces desmosome stability. J. Cell Sci. 116, 3303–3314 (2003). human WB
Akat, K. et al. Molecular characterization of desmosomes in meningiomas and arachnoidal tissue. Acta Neuropathol. 106, 337–347 (2003). human WB, IHC-IF
Mertens, C., Kuhn, C., Moll, R., Schwetlick, I. & Franke, W. W. Desmosomal plakophilin 2 as a differentiation marker in normal and malignant tissues. Differentiation 64, 277–290 (1999). human, bovine IHC (frozen), ICC-IF
Mertens, C., Kulm, C. & Franke, W. W. Plakophilins 2a and 2b: Constitutive Proteins of Dual Location in the Karyoplasm and the Desmosomal Plaque. J. Cell Biol. 135, 1009–1025 (1996). human, bovine IHC (frozen), ICC-IF

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