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IHC of human colon (courtesy of J.Heß, University Hospital Heidelberg) Zoom

IHC of human colon (courtesy of J.Heß, University Hospital Heidelberg)

anti-Plakophilin 2 mouse monoclonal, PP2/62 + PP2/86 + PP2/150, supernatant

Plakophilin 2 is a desmosomal plaque protein in simple and glandular epithelia, basal layer of stratified epithelia and in some nonepithelial tissues, e.g. myocard; in some cells devoid of desmosomes, PP2 is enriched in the karyoplasm.
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Cat. No.: 651101
Quantity:  5 mL

Delivery Time: usually 1-7 working days

$304.00
Excl. 19% Tax, excl. Shipping Cost

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Specifications

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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone PP2/62 + PP2/86 + PP2/150
Immunogen synthetic carboxyterminal peptide (24 aa) of human plakophilin 2
Purification hybridoma cell culture supernatant
Conjugate unconjugated
Formulation contains 0.09% sodium azide
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Intended use Research use only
Tested species reactivity bovine, human, rat

Applications

Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen 1:2 - 1:50 (incubate for 5 min in PBS plus 0.2% Triton-X100 prior to antibody application; longer incubation could result in a loss of nuclear staining; fixation with 2% formaldehyde is recommended; exclusive nuckear staining without detergent)
Immunohistochemistry (IHC) - paraffin 1:2 - 1:50 (microwave treatment recommended)
Immunoprecipitation (IP) assay dependent
Western Blot (WB) assay dependent

Background

Details

The mabs react with plakophilin 2, a member of the arm-repeat family of proteins which can occur in at least 2 splice forms (2a and 2b). Plakophilin 2 assembles with other proteins to form the desmosomal plaque in simple and glandular epithelia, the basal layer of certain stratified epithelia, all layers of some stratified epithelia, and in nonepithelial desmosome-possessing tissues such as myocardium, Purkinje fibers, and dendritic reticulum of lymph node follicles. In all these cells, including a variety of cell types devoid of desmosomes, plakophilin 2 is highly enriched in the karyoplasm. Polypeptide reacting: Plakophilin 2, Mr 100 kD (SDS-PAGE); MW calculated from aa sequence: 92,750 (pI 9.33) and 97,410 (pI 9.38)

Tumors specifically detected: Positive staining was obtained with several carcinomas derived from simple-type epithelia (e.g. hepatomas, colon carcinomas) and some adenocarcinomas derived from stratified epithelia (oesophagus carcinoma). The antibody was negative with all leiomyosarcomas tested.

Reference

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