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anti-Plakophilin 3 mouse monoclonal, 310.9.1, supernatant

The anti-plakophilin 3 antibody stains plakophilin 3, seen in most desmosomes and partly dispersed in the cytoplasm.
Cat. No.: 651114
Quantity:  5 mL

Delivery Time: usually 1-7 working days

€196.00
Excl. 19% Tax, excl. Shipping Cost

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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone 310.9.1
Immunogen recombinant protein (E. coli) of human plakophilin
Purification hybridoma cell culture supernatant
Conjugate unconjugated
Formulation contains 0.09% sodium azide
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Tested species reactivity bovine, human, mouse, Xenopus laevis

Applications

Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen ready-to-use
Western Blot (WB) 1:1,000

Background

Details

Plakophilin 3 is seen in most desmosomes and partly dispersed in cytoplasm under various fixation conditions (FA, MeAc long, MeAc short):

Positive tissues and culture cells:

  • stratified squamous epithelia: palmar, epidermis, scalp skin
  • stratified epithelia: tongue, vagina, esophagus
  • transitional epithelia: bladder
  • non-stratified epithelia:  colon, duodenum, pancreas, several glands
  • non-epithelial: reticulum cells of lymph node follicle
  • HaCaT
  • MCF7, HeLa, HAT-29, CaCo 2, Caski, BMGE, MDCK and other

Negative tissues and cell lines:

  • liver, heart, skeletal muscle, endothelia (vessels)
  • PLC, RD, SV80, HL60, Raji, Jok1, Glioma, K562, 3T3L1, A6, PtK2

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