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anti-Plectin guinea pig polyclonal, serum

Localizing to plasma membrane attachment sites of intermediate filaments and microfilaments (actin), such as desmosomes and hemidesmosomes, Z-line structures and dense plaques of striated and smooth muscle.
Cat. No.: GP21
Quantity:  100 µL

Delivery Time: usually 1-7 working days

€249.00
Excl. 19% Tax, excl. Shipping Cost

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Product description

Host guinea pig
Antibody Type polyclonal
Immunogen C-terminal “C” domain of human recombinant Plectin (aa 4367 - 4684)
Purification stabilized antiserum
Conjugate unconjugated
Formulation contains 0.09% sodium azide and 0.5% BSA
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
UniprotID Q15149
Tested species reactivity bovine, fish, human, mouse, Xenopus laevis

Applications

Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) assay dependent
Immunohistochemistry (IHC) - frozen 1:500
Immunohistochemistry (IHC) - paraffin 1:500 (microwave treatment recommended)
Western Blot (WB) 1:16,000

Background

Details

Calculated MW 532,000 protein plectin, localizing to plasma membrane attachment sites of intermediate filaments and microfilaments (actin) such as desmosomes and hemidesmosomes, Z-line structures and dense plaques of striated and smooth muscle. Strong positive reaction is also found with endothelia, fibro-blasts and infiltrating cells (e.g. lymphocytes). Human glia cells are specifically detected.

Reactivity on cultured cell lines: HL-60, Huvec, BMGE, 3T3

Reference

Reference

PublicationSpeciesApplication Schröder, R. et al. Immunogold EM reveals a close association of plectin and the desmin cytoskeleton in human skeletal muscle. Eur. J. Cell Biol. 78, 288–95 (1999). human, mouse, bovine WB, IHC (frozen)

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