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anti-Pneumocystis jirovecii mouse monoclonal, PRO225, lyophilized, purified

The antibody reacts specifically with P. jirovecii (carinii) and stains cysts as well as trophozoites. In Western blotting analysis the antibody identifies 2 antigens at 65/67 kD of P. jirovecii (carinii).
Cat. No.: 610225
Quantity:  100 µg
No. of experiments:  ≤ 400 (IHC, 50 µl/slide)

Delivery Time: usually 1-7 working days

$245.00
Excl. VAT, excl. Shipping Cost

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Specifications

Product description

Host mouse
Antibody Type monoclonal
Isotype IgG2b
Clone PRO225
Immunogen Pneumocystis jiroveci (P. carinii) isolates
Purification protein A affinity chromatography
Conjugate unconjugated
Formulation lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09 % sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
Storage short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles
No. of experiments ≤ 400 (IHC, 50 µl/slide)
Intended use Research use only
Tested species reactivity P. carinii

Applications

Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) 1:20
Immunohistochemistry (IHC) - frozen 1:20
Western Blot (WB) assay dependent

Background

Details

PRO225 antibody reacts specifically with P. jiroveci (P. carinii) pneumonia, staining cysts as well as trophozoite present in biopsy material, bronchial lavage and induced sputum samples. For bronchial lavage and induced sputum samples pretreatment of samples with 0.3% dithiothreitol or sputolysin is recommended.
In Western blotting analysis the antibody identifies 2 antigens at 65/67 kD of P. jiroveci (P. carinii).
The antibody is also reactive with P. jiroveci (P. carinii) isolates from rat.

Reference

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