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anti-Pneumocystis jirovecii mouse monoclonal, PRO226, lyophilized, purified

The antibody reacts specifically with P. jirovecii (carinii) and stains cysts as well as trophozoites. In Western blotting analysis the antibody identifies an antigen at 50 kD of P. jirovecii (carinii).
Cat. No.: 610226
Quantity:  100 µg

Delivery Time: usually 1-7 working days

€196.00
Excl. 19% Tax, excl. Shipping Cost

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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG3
Clone PRO226
Immunogen Pneumocystis jiroveci (P. carinii) isolates
Purification protein A affinity chromatography
Conjugate unconjugated
Formulation lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09 % sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Tested species reactivity P. carinii

Applications

Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) 1:20
Western Blot (WB) assay dependent

Background

Details

PRO226 antibody reacts specifically with P. jiroveci (P. carinii) pneumonia, staining cysts as well as trophozoite present in biopsy material, bronchial lavage and induced sputum samples. For bronchial lavage and induced spu-tum samples pretreatment of samples with 0.3% dithio-threitol or sputolysin is recommended.
In Western blotting analysis the antibody identifies an an-tigen of 50 kD of P. jiroveci (P. carinii).
The antibody is also reactive with P. jiroveci (P. carinii) isolates from rat.

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