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anti-Pneumocystis jirovecii mouse monoclonal, PRO227, lyophilized, purified

The antibody reacts specifically with P. jirovecii (carinii) and stains cysts. In Western blotting analysis the antibody identifies an antigen at 93 kD of P. jirovecii (carinii).
Cat. No.: 610227
Quantity:  100 µg
No. of experiments:  ≤ 400 (IHC, 50 µl/slide)

Delivery Time: usually 1-7 working days

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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG2b
Clone PRO227
Immunogen Pneumocystis jiroveci (P. carinii) isolates
Purification protein A affinity chromatography
Conjugate unconjugated
Formulation lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09 % sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
Storage short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles
No. of experiments ≤ 400 (IHC, 50 µl/slide)
Intended use Research use only
Tested species reactivity P. carinii


Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) 1:20
Immunohistochemistry (IHC) - frozen 1:20
Western Blot (WB) assay dependent



PRO227 antibody reacts specifically with P. jiroveci (P. carinii) pneumonia, staining especially cysts present in biopsy material, bronchial lavage and induced sputum samples. For bronchial lavage and induced sputum samples pretreatment of samples with 0.3% dithiothreitol or sputolysin is recommended.
In Western blotting analysis the antibody identifies an antigen of  93 kD of P. jiroveci (P. carinii).


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