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Product description
| Host | guinea pig |
|---|---|
| Antibody Type | polyclonal |
| Immunogen | synthetic C-terminal peptide of human Prp19p protein (aa 182 – 192) conjugated to KLH |
| Purification | stabilized antiserum |
| Conjugate | unconjugated |
| Formulation | contains 0.09% sodium azide and 0.5% BSA |
| Storage | short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles |
| No. of experiments | ≤ 400 (IHC, 50 µl/slide); ≤ 40 (WB, 5 ml reaction volume) |
| Note | centrifuge prior to opening |
| Intended use | Research use only |
| Tested species reactivity | human, mouse |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Immunohistochemistry (IHC) - frozen | 1:100 - 1:200 |
| Western Blot (WB) | 1:2,000 |
Background
Details
Prp19p was described to be a lipid droplet-associated protein (Cho et al., 2007). However, it does not belong to the PLIN/PAT familily of lipid droplet-associated proteins and preferentially is a member of the spliceosomal complex.
In addition Prp19p protein was also found in the nuclei of cultured cells (Ohi et al., 2005).
Reactivity on cultured cell lines: PLC, SV80.
Cho SY, Shin ES, Park PJ, Shin DW, Chang HK, Kim D, Lee HH, Lee JH, Kim SH, Song MJ, Chang IS, Lee OS, Lee TR: Identification of mouse Prp19p as a lipid droplet-associated protein and its possible involvement in the biogenesis of lipid droplets. J Biol Chem 282, 2456-2465 (2007).
Ohi MD, Vander Kooi CW, Rosenberg JA, Ren L, Hirsch JP, Chazin WJ, Walz T, Gould KL: Structural and functional analysis of essential pre-mRNA splicing factor Prp19p. Mol Cell Biol 25, 451-460 (2005).
Reference
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