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Product description
| Host | mouse |
|---|---|
| Antibody Type | monoclonal |
| Isotype | IgG1 |
| Clone | ARNA-3 |
| Immunogen | purified drosophila melanogaster RNA polymerase II |
| Purification | hybridoma cell culture supernatant concentrate |
| Conjugate | unconjugated |
| Formulation | contains 0.09% sodium azide |
| Storage | short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles |
| No. of experiments | ≤ 200 (IHC, 50 µl/slide) |
| Note | centrifuge prior to opening |
| Intended use | Research use only |
| Tested species reactivity | drosophila, human |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Immunocytochemistry (ICC)/ Immunofluorescence (IF) | assay dependent (recommended fixation conditions for cells: incubate in 2% paraformaldehyde in PBS for 10 min, wash in PBS for 5 min, incubate in 0.2% Triton X-100 in PBS for 5 min, wash in PBS for 5 min) |
| Immunohistochemistry (IHC) - frozen | 1:100 |
| Western Blot (WB) | assay dependent |
Background
Details
ARNA-3 is excellent for detection of transcription activity, e.g. puffs in polytene chromosomes. Reacts with RNA polymerase II (Mr 175,000 subunit of RNAP II) in biological materials. Reactive polypeptides after SDS-PAGE: 200 kD band of nonphosphorylated and 240 kD band of phosphorylated Pol IIA.
Epitope recognized within: 794-DDYGPESRGFVENSYLA-822
Reference
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