You have no items in your shopping cart.
Product description
Host | mouse |
---|---|
Antibody Type | monoclonal |
Isotype | IgG1 |
Clone | ARNA-3 |
Immunogen | purified drosophila melanogaster RNA polymerase II |
Purification | hybridoma cell culture supernatant concentrate |
Conjugate | unconjugated |
Formulation | contains 0.09% sodium azide |
Storage | short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles |
No. of experiments | ≤ 200 (IHC, 50 µl/slide) |
Note | centrifuge prior to opening |
Intended use | Research use only |
Tested species reactivity | drosophila, human |
Applications
Tested applications | Tested dilutions |
---|---|
Immunocytochemistry (ICC)/ Immunofluorescence (IF) | assay dependent (recommended fixation conditions for cells: incubate in 2% paraformaldehyde in PBS for 10 min, wash in PBS for 5 min, incubate in 0.2% Triton X-100 in PBS for 5 min, wash in PBS for 5 min) |
Immunohistochemistry (IHC) - frozen | 1:100 |
Western Blot (WB) | assay dependent |
Background
Details
ARNA-3 is excellent for detection of transcription activity, e.g. puffs in polytene chromosomes. Reacts with RNA polymerase II (Mr 175,000 subunit of RNAP II) in biological materials. Reactive polypeptides after SDS-PAGE: 200 kD band of nonphosphorylated and 240 kD band of phosphorylated Pol IIA.
Epitope recognized within: 794-DDYGPESRGFVENSYLA-822
Reference
Filter
Publication
Species
Application