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anti-RNA Polymerase II mouse monoclonal, ARNA-3, supernatant concentrate

ARNA-3 is excellent for the detection of transcription activity. Reacts with Mr 175,000 subunit of RNAP II. Epitope recognized: 794-DDYGPESRGFVENSYLA-822.
Cat. No.: 65223
Quantity:  100 µL
No. of experiments:  ≤ 200 (IHC, 50 µl/slide)

Delivery Time: usually 1-7 working days

$258.00
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Specifications

Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone ARNA-3
Immunogen purified drosophila melanogaster RNA polymerase II
Purification hybridoma cell culture supernatant concentrate
Conjugate unconjugated
Formulation contains 0.09% sodium azide
Storage short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles
No. of experiments ≤ 200 (IHC, 50 µl/slide)
Note centrifuge prior to opening
Intended use Research use only
Tested species reactivity drosophila, human

Applications

Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) assay dependent (recommended fixation conditions for cells: incubate in 2% paraformaldehyde in PBS for 10 min, wash in PBS for 5 min, incubate in 0.2% Triton X-100 in PBS for 5 min, wash in PBS for 5 min)
Immunohistochemistry (IHC) - frozen 1:100
Western Blot (WB) assay dependent

Background

Details

ARNA-3 is excellent for detection of transcription activity, e.g. puffs in polytene chromosomes. Reacts with RNA polymerase II (Mr 175,000 subunit of RNAP II) in biological materials. Reactive polypeptides after SDS-PAGE: 200 kD band of nonphosphorylated and 240 kD band of phosphorylated Pol IIA.
Epitope recognized within: 794-DDYGPESRGFVENSYLA-822

Reference

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