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anti-RNA Polymerase II mouse monoclonal, ARNA-3, supernatant

ARNA-3 is excellent for the detection of transcription activity. Reacts with Mr 175,000 subunit of RNAP II. Epitope recognized: 794-DDYGPESRGFVENSYLA-822
Cat. No.: 65123
Quantity:  5 mL

Delivery Time: usually 1-7 working days

€139.00
Excl. 19% Tax, excl. Shipping Cost

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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone ARNA-3
Immunogen Purified RNA polymerase II
Purification hybridoma cell culture supernatant
Conjugate unconjugated
Formulation contains 0.09% sodium azide
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Tested species reactivity human, insects

Applications

Immunocytochemistry (ICC)/ Immunofluorescence (IF) ready-to-use (recommended fixation: 10 min 2% paraformaldehyde in PBS, wash in PBS for 5 min, 5 min 0.2% Triton X-100 in PBS, wash in PBS for 5 min)
Immunohistochemistry (IHC) - frozen ready-to-use (recommended fixation: 10 min 2% paraformaldehyde in PBS, wash in PBS for 5 min, 5 min 0.2% Triton X-100 in PBS, wash in PBS for 5 min)
Western Blot (WB) assay dependent

Background

Details

ARNA-3 is excellent for detection of transcription activity, e.g. puffs in polytene chromosomes. Reacts with RNA polymerase II (Mr 175,000 subunit of RNAP II) in biological materials. Reactive polypeptides after SDS-PAGE: 200 kD band of nonphosphorylated and 240 kD band of phosphorylated Pol IIA.           
Epitope recognized: 794DDYGPESRGFVENSYLA822.

Reactivity on cultured cell lines: Drosophila chromosomes, PTK-2, HeLa, Hep2.

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