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anti-S100 Protein rabbit polyclonal, serum

Reacts with the 21-24 kD A and B subunits of the S100 protein. Stains schwannomas, ependymomas, astrogliomas and almost all melanomas. Langerhans cells in skin and some reticulum cells in the paracortex of lymph nodes are positive.
Cat. No.: 16100
Quantity:  250 µL
No. of experiments:  ≤ 500 (IHC, 50 µl/slide)

Delivery Time: usually 1-7 working days

$285.00
Excl. VAT, excl. Shipping Cost

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Specifications

Product description

Host rabbit
Antibody Type polyclonal
Immunogen S-100 protein from bovine brain
Purification stabilized antiserum
Conjugate unconjugated
Formulation contains 0.09% sodium azide
Storage short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles
No. of experiments ≤ 500 (IHC, 50 µl/slide)
Note centrifuge prior to opening
Intended use Research use only
Tested species reactivity bovine, human

Applications

Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen 1:50 – 1:100
Immunohistochemistry (IHC) - paraffin 1:50 – 1:100 (microwave treatment recommended)

Background

Details

Reacts with the 21 - 24 kD (the A and B subunits) of the S-100 protein. S-100A is composed of one alpha- and one beta-chain, whereas S-100B is composed of two beta-chains. S-100 is a brain protein normally present in astrocytes, melanocytes, ependymal cells, glial cells and Schwann cells. Langerhans cells in the skin and interdigitating reticulum cells in the paracortex of lymph nodes also stain positively. S-100 occurs in tumors of the central nervous system, such as Schwannomas, ependymomas, astrogliomas, gliastomas and almost all melanomas.

Positive control: human skin.

Reference

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