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anti-Symplekin mouse monoclonal, Sym-TJ-E150, supernatant

Sym-TJ-E150 reacts with a 150 kD polypeptide in tight junctions (zonula occludens) of polar epithelia and Sertoli cells of testis, and in the nucleoplasm of cells, which do not form tight junctions.
Cat. No.: 651100
Quantity:  5 mL

Delivery Time: usually 1-7 working days

€196.00
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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone Sym-TJ-E150
Immunogen human symplekin
Purification hybridoma cell culture supernatant
Conjugate unconjugated
Formulation contains 0.09% sodium azide
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Tested species reactivity human, rat (negative with bovine, mouse, Xenopus laevis)

Applications

Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen ready-to-use (for nucleoplasmic staining a modified Carnoy fixation procedure is suggested (60% ethanol, 30% chloroform, 10% acetic acid) for 20 min, followed by a 10 min ethanol wash)
Immunohistochemistry (IHC) - paraffin ready-to-use (microwave treatment recommended; for nucleoplasmic staining a modified Carnoy fixation procedure is suggested (60% ethanol, 30% chloroform, 10% acetic acid) for 20 min, followed by a 10 min ethanol wash)
Western Blot (WB) assay dependent

Background

Details

The mab reacts specifically with symplekin (a 150 kDa polypeptide) present in 1) tight junctions (zonula occludens) of polar epithelia and Sertoli cells of testis (but not their vascular endothelia!) and 2) exclusively in the nucleoplasm of these and many other cell types which do not form tight junctions (e.g. stratified epithelia such as oesophagus, tongue mucosa, epidermis; squamous cell carcinoma; non-epithelia such as fibroblasts, endothelia, lymphocytes).

Polypeptide reacting: Symplekin, 126.5 kD polypeptide (calculated from aa sequence data); apparent Mr 150 kD (after SDS-PAGE); pI 6.5.

Reactivities on Cultured Cell lines: Carcinoma cell lines (e.g. MCF-7, Caco-2, HT29, PLC), SV80 (fibroblasts), U333 CG / 343 MG (glioma), K 562 (erythroleukemia), RVF (rat vascular smooth muscle cells).

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