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mouse brain (courtesy of J.Heß, University Hospital Heidelberg) Zoom

mouse brain (courtesy of J.Heß, University Hospital Heidelberg)

anti-Synaptophysin mouse monoclonal, SY38, lyophilized, purified

The anti-synaptophysin antibody SY38 stains neuronal and adrenal tumors such as pheochromocytomas, paragangliomas, neuroblastomas and neuroendocrine tumors of epithelial origin, e.g. pancreatic islet cell carcinoma, bronchial and gastrointestinal carcinoids.
highly_published external_validation
Cat. No.: 61012
Quantity:  50 µg
No. of experiments:  ≤ 1000 (IHC, 50 µl/slide)

Delivery Time: usually 1-7 working days

$281.00
Excl. VAT, excl. Shipping Cost

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Specifications

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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone SY38
Immunogen synaptophysin from presynaptic vesicles, prepared from bovine brain
Purification protein A affinity chromatography
Conjugate unconjugated
Formulation lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09 % sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
Storage short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles
UniprotID P20488
No. of experiments ≤ 1000 (IHC, 50 µl/slide)
Intended use Research use only
Tested species reactivity bovine, human, mouse, rat

Applications

Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) assay dependent
Immunohistochemistry (IHC) - frozen at least 1:50 with PBS, pH 7.4 (no protease treatment)
Immunohistochemistry (IHC) - paraffin at least 1:50 with PBS, pH 7.4 (microwave treatment recommended, no protease treatment)
Western Blot (WB) assay dependent

Background

Details

SY 38 represents an excellent marker for several neuroendocrine, neuronal and adrenal tumors. Neuronal and adrenal tumors such as pheochromocytomas, paragangliomas, neuroblastomas, ganglioneuroblastomas. Neuroendocrine tumors of epithelial origin: Pancreatic islet cell carcinoma, bronchial and gastrointestinal carcinoids, medullary carcinoma of thyroid. Polypeptide reacting: Mr 38 000 transmembrane glycoprotein of presynaptic vesicles.

Tested cultured cell lines: Rat PC-12 cell line

Reference

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