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Synaptopodin staining in human glomeruli (GP94-N; dilution 1:50;  Image courtesy of A.Weins, Brigham and Women's Hospital)	Zoom

Synaptopodin staining in human glomeruli (GP94-N; dilution 1:50; Image courtesy of A.Weins, Brigham and Women's Hospital)

anti-Synaptopodin (N-terminus) guinea pig polyclonal, serum

The antibody reacts specifically with the N-terminal region of synaptopodin and recognizes differentiated podocytes (glomerular visceral epithelial cells) in vivo and in vitro (weaker additional reaction with arterial endothelial cells), co-localization with alpha-actinin. GP94-N also reacts with a subset of exclusively telencephalic synapses.
Cat. No.: GP94-N
Quantity:  100 µL

Delivery Time: usually 1-7 working days

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Product description

Host guinea pig
Antibody Type polyclonal
Immunogen Synthetic peptides (mouse N-terminus), coupled to KLH
Purification stabilized antiserum
Conjugate unconjugated
Formulation contains 0.09% sodium azide and 0.5% BSA
Storage store at 2 – 8 °C
Tested species reactivity human, mouse

Applications

Immunohistochemistry (IHC) - frozen 1:50 – 1:100
Immunohistochemistry (IHC) - paraffin 1:50 – 1:100 (microwave treatment recommended)
Western Blot (WB) 1:500 -1:1,000

Background

Details

The antibody reacts specifically with the N-terminus of synaptopodin, a prolin-rich actin-binding protein with 2 binding sites for actin. Synaptopodin belongs to actin-binding pro-teins, it has first been localized in podocytes and a subset of telencephalic postsynaptic densities. In human tissue synaptopodin has a molecular weight of 73.7 kD and pI of 9.38 (calculated from sequence data); in mouse the corre-sponding data are 74 kD, pI 9.27. In SDS-PAGE the antigen appears as 100 kD polypeptide in brain and 110 kD polypeptide in kidney (attributed to posttransla-tional modifications). In Western blot analysis the antibody also reacts with a 44 kD degradation fragment of synaptopodin.

The antibody recognizes differentiated podocytes (glomerular visceral epithelial cells) in vivo and in vitro (weaker additional reaction with arterial endothelial cells), co-localization with alpha-actinin.

Reacts with a subset of exclusively telencephalic synapses. Differentiation-dependent expression during postnatal maturation of murine brain. Differentiation-dependent expression in cultured hippocampal neurons.

Reference

Reference

  1. Mundel P, Gilbert P, and W Kriz. Podocytes in Glomerulus of Rat Kidney Express a Characteristic 44 kD Protein. J Histochem and Cytochem Vol 39 No 8:1047-1056 (1991)
  2. Mundel P and W Kritz. Structure and function of podocytes: an update. Anat Embryol 192:385-397 (1995)
  3. Mundel P, Heid HW, Mundel TM, Krüger M, Reiser J, Kriz W. Synaptopodin, an actin-associated protein in telencephalic dendrites and in renal podocytes. J Cell Biol Vol 139 (1):193-204 (1997)
  4. Asanuma K, Kim K, Oh J, Giardino L, Chabanis S, Faul C, Reiser J, Mundel P: synaptopodin regulates the actin-bundling activity of a-actinin in an isoform-specific manner. J Clin Invest 115, 1188-1198 (2005)
  5. Zhang XL, Pöschel B, Faul C, Upreti C, Stanton PK, Mundel P: Essential role for synaptopodin in dendritic spine plasticity of the developing hippocampus. J Neurosci 2013 Jul 24;33(30):12510-8. doi: 10.1523/JNEUROSCI.2983-12.201

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