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rat kidney (courtesy of J. Heß, University Hospital Heidelberg) Zoom

rat kidney (courtesy of J. Heß, University Hospital Heidelberg)

anti-Synaptopodin/SYNPO mouse monoclonal, G1D4, lyophilized, purified

The anti-synaptopodin/SYNPO antibody recognizes differentiated glomerular visceral epithelial cells (podocytes) in vivo and in vitro. Weaker additional reaction with arterial endothelial cells.
highly_published external_validation
Cat. No.: 61094
Quantity:  50 µg
No. of experiments:  ≤ 200 (IHC, 50 µl/slide)

Delivery Time: usually 1-7 working days

$364.00
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Specifications

Alternative Products

Cat. No. Product Name Format Quantity Price
65194

anti-Synaptopodin/SYNPO mouse monoclonal, G1D4

supernatant 5 mL
$251.00
Excl. VAT, excl. Shipping Cost

Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone G1D4
Immunogen Isolated rat kidney glomeruli
Purification protein A affinity chromatography
Conjugate unconjugated
Formulation lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09 % sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
Storage short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles
UniprotID Q9Z327
No. of experiments ≤ 200 (IHC, 50 µl/slide)
Intended use Research use only
Tested species reactivity human, mouse, rat (negative with rabbit, frog, chicken)

Applications

Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) assay dependent
Immunohistochemistry (IHC) - frozen 1:50 – 1:200
Immunohistochemistry (IHC) - paraffin 1:50 – 1:200 (microwave treatment recommended)
Western Blot (WB) assay dependent

Background

Details

The antibody recognizes differentiated podocytes (glomerular visceral epithelial cells) in vivo and in vitro (weaker additional reaction with arterial endothelial cells), co-localization with α-actinin. Does not react with parietal cells. Reacts with a subset of exclusively telencephalic synapses. Differentiation-dependent expression during postnatal maturation of rat brain. Differentiation-dependent expression in cultured hippocampal neurons. The antibody reacts specifically with synaptopodin, a prolin-rich actin-binding protein with 2 binding sites for actin. Synaptopodin represents a new class of actin-binding proteins which has first been localized in podocytes and a subset of telencephalic postsynaptic densities. In human tissue synaptopodin has a molecular weight of 73.7 kD and pI of 9.38 (calculated from sequence data); in mouse the corresponding data are 74 kD, pI 9.27. In SDS-PAGE the antigen appears as 100 kD polypeptide in brain and 110 kD polypeptide in kidney (the difference might be attributed to posttranslational modifications). In Western blot analysis the antibody also reacts with a 44 kD degradation fragment of synaptopodin.

Reference

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