Product description
Host | guinea pig |
---|---|
Antibody Type | polyclonal |
Immunogen | synthetic peptides (mouse N-terminus), coupled to KLH |
Purification | stabilized antiserum |
Conjugate | unconjugated |
Formulation | contains 0.09% sodium azide and 0.5% BSA |
Storage | short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles |
No. of experiments | ≤ 200 (IHC, 50 µl/slide); ≤ 20 (WB, 5 ml reaction volume) |
Note | centrifuge prior to opening |
Intended use | Research use only |
Tested species reactivity | human, mouse |
Applications
Tested applications | Tested dilutions |
---|---|
Immunocytochemistry (ICC)/ Immunofluorescence (IF) | assay dependent |
Immunohistochemistry (IHC) - frozen | 1:50 – 1:100 |
Immunohistochemistry (IHC) - paraffin | 1:50 – 1:100 (microwave treatment recommended) |
Western Blot (WB) | 1:500 -1:1,000 |
Background
Details
The antibody reacts specifically with the N-terminus of synaptopodin/SYNPO, a prolin-rich actin-binding protein with 2 binding sites for actin. Synaptopodin belongs to actin-binding pro-teins, it has first been localized in podocytes and a subset of telencephalic postsynaptic densities. In human tissue synaptopodin has a molecular weight of 73.7 kD and pI of 9.38 (calculated from sequence data); in mouse the corre-sponding data are 74 kD, pI 9.27. In SDS-PAGE the antigen appears as 100 kD polypeptide in brain and 110 kD polypeptide in kidney (attributed to posttransla-tional modifications). In Western blot analysis the antibody also reacts with a 44 kD degradation fragment of synaptopodin.
The antibody recognizes differentiated podocytes (glomerular visceral epithelial cells) in vivo and in vitro (weaker additional reaction with arterial endothelial cells), co-localization with alpha-actinin.
Reacts with a subset of exclusively telencephalic synapses. Differentiation-dependent expression during postnatal maturation of murine brain. Differentiation-dependent expression in cultured hippocampal neurons.