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Product description
| Host | mouse |
|---|---|
| Antibody Type | monoclonal |
| Isotype | IgG2a kappa |
| Clone | VW-1B1-A8-F4 |
| Immunogen | HPLC purified peptide from TKT |
| Purification | affinity chromatography |
| Conjugate | unconjugated |
| Formulation | lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09 % sodium azide, 0.5% BSA in PBS buffer, pH 7.4) |
| Storage | short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles |
| No. of experiments | ≤ 1000 (IHC, 50 µl/slide) |
| Intended use | Research use only |
| Tested species reactivity | human |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Immunocytochemistry (ICC)/ Immunofluorescence (IF) | assay dependent |
| Immunohistochemistry (IHC) - frozen | 1:50 |
| Western Blot (WB) | assay dependent |
Background
Details
Transketolase dependent reactions regulate the non-oxidative part of the pentose-phosphate-pathway (PPP) in glucose metabolism. This pathway generates NADPH and pentose phosphates. The latter are important for nucleic acid metabolism. Beside the ubiquitous Transketolase (TKT) other Transketolase-like proteins exist.
Reactivity on cultured cell lines: HepG2.
Reference
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