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anti-TKTL1 (Transketolase-like 1) mouse monoclonal, VU-7H2-G5-C1, lyophilized, purified

Publications suggest an important role of TKTL1 in the metabolism of tumor cells. In colorectal tumors nuclear as well as cytoplasmic localization has been described.
Cat. No.: 610175
Quantity:  50 µg
No. of experiments:  ≤ 1000 (IHC, 50 µl/slide); ≤ 40 (WB, 5 ml reaction volume)

Delivery Time: usually 1-7 working days

$278.00
Excl. VAT, excl. Shipping Cost

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Specifications

Product description

Host mouse
Antibody Type monoclonal
Isotype IgG2a kappa
Clone VU-7H2-G5-C1
Immunogen HPLC purified peptide from TKTL1
Purification protein A affinity chromatography
Conjugate unconjugated
Formulation lyophilized; reconstitute in 1 mL dist. water (final solution contains 0.09% sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
Storage short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles
No. of experiments ≤ 1000 (IHC, 50 µl/slide); ≤ 40 (WB, 5 ml reaction volume)
Intended use Research use only
Tested species reactivity human

Applications

Tested applications Tested dilutions
Immunohistochemistry (IHC) - paraffin 1:50 (perform heat-induced epitope retrieval by heating the paraffin sections at 95 °C for 30 min in 10 mM citrate buffer (pH 6.0)
Western Blot (WB) 1:200

Background

Details

Expression studies on transketolase-like-1 show that the protein is found in aggressive tumors. The detection of TKTL1 is furthermore correlated with poor patient prognosis and metastasis. Transketolase dependent reactions regulate the non-oxidative part of the pentose-phosphate-pathway (PPP) in glucose metabolism. This pathway generates NADPH and pentose phosphates. The latter are important for nucleic acid metabolism. Beside the ubiquitous transketolase (TKT) other transketolase-like proteins exist. Transketolase-like 1 (TKTL1) is a protein with a 38 amino acid deletion when compared to TKT. TKTL1 expression has been shown in tumors of different entities. Inhibition of TKTL1 expression by RNA interference results in reduced growth of tumor cells, while overexpression of TKTL1 promotes cell proliferation. Recent publications suggest an important role of TKTL1 in the metabolism of tumor cells. In colorectal tumors nuclear as well as cytoplasmic localization has been described.

Reactivity on cultured cell lines: THP-1.

Reference

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