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human Melanoma (frozen section) Zoom

human Melanoma (frozen section)

anti-Transferrin Receptor mouse monoclonal, PAL-M1, supernatant

Discriminates melanomas from nevocellular nevi. Stains most of the primary melanomas and metastases derived therefrom. Excellent marker for neoplastic progression of melanocytic proliferation.
Cat. No.: 10822
Quantity:  1 mL
No. of experiments:  ≤ 200 (IHC, 50 µl/slide)

Delivery Time: usually 1-7 working days

$215.00
Excl. VAT, excl. Shipping Cost

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Specifications

Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone PAL-M1
Immunogen isolated from human melanoma cells
Purification hybridoma cell culture supernatant
Conjugate unconjugated
Formulation contains 0.09% sodium azide
Storage short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles
No. of experiments ≤ 200 (IHC, 50 µl/slide)
Note centrifuge prior to opening
Intended use Research use only
Tested species reactivity human, mouse

Applications

Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen 1:5 - 1:10

Background

Details

Can be used to discriminate between malignant cutaneous melanoma and nevocellular nevi. PAL-M1 recognizes the transferrin receptor (TFR, CD71), a marker for tumor progression in the melanocytic lineage and stains the majority of primary melanomas and their metastases. Local staining of dysplastic nevocellular nevi may be observed. Common nevocel­lular nevi rarely stain. Excellent marker for neoplastic progression of melanocytic proliferation.
Reactivity on cultured cell lines: Melanoma cell lines Mel 57, CM 136, M14, BLM; HepG2, PLC, Daudi, Raji, PBL

Positive control: Human Melanoma.

Reference

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