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anti-Tubulin mouse monoclonal, 655.0, supernatant

Mab 655 specifically stains tubulin in immunohistochemistry and reacts in ELISA and WB.
Cat. No.: 10610
Quantity:  1 mL
No. of experiments:  ≤ 200 (IHC, 50 µl/slide)

Delivery Time: usually 1-7 working days

$306.00
Excl. VAT, excl. Shipping Cost

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Specifications

Product description

Host mouse
Antibody Type monoclonal
Isotype IgM
Clone 655
Immunogen Isolated from chicken skeletal muscle preparation
Purification hybridoma cell culture supernatant
Conjugate unconjugated
Formulation contains 0.09% sodium azide
Storage short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles
No. of experiments ≤ 200 (IHC, 50 µl/slide)
Note centrifuge prior to opening
Intended use Research use only
Tested species reactivity chicken, human

Applications

Tested applications Tested dilutions
ELISA assay dependent
Immunocytochemistry (ICC)/ Immunofluorescence (IF) 1:5 - 1:10
Immunohistochemistry (IHC) - frozen 1:5 - 1:10
Immunohistochemistry (IHC) - paraffin 1:5 - 1:10 (microwave treatment recommended)
Western Blot (WB) assay dependent

Background

Details

Tubulin is found in the microtubules which represent the main structural components of centriols, the mitotic spindle, cilia and flagella. Mab 655 specifically stains tubulin in immunohistochemistry (staining of microtubules abundantly present in muscle and non-muscle cells) and reacts in ELISA and WB.
The antibody cross reacts with human tissue.
Positive control: Cultured fibroblasts (chicken)

Reference

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