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Product description
| Host | guinea pig |
|---|---|
| Antibody Type | polyclonal |
| Immunogen | Synthetic peptides common to human type I (acidic) and type II (basic) hair (trichocytic) keratins K31-K40 (former designation hHa1-hHa8, Ka35 and Ka36): LESEDCKLPSNP-C; and K81-K86 (former designation hHb1-hHb4): VNVCVSSSRGGVV-C, both coupled to KLH |
| Purification | stabilized antiserum |
| Conjugate | unconjugated |
| Formulation | contains 0.09% sodium azide and 0.5% BSA |
| Storage | short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles |
| No. of experiments | ≤ 200 (IHC, 50 µl/slide) |
| Note | centrifuge prior to opening |
| Intended use | Research use only |
| Tested species reactivity | human, mouse |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Immunohistochemistry (IHC) - frozen | 1:100 |
| Immunohistochemistry (IHC) - paraffin | 1:100 (microwave treatment recommended) |
| Western Blot (WB) | assay dependent |
Background
Details
The antiserum stains most prominently human hair keratins K81-K86 (type II) and considerably weaker K31-K40 (type I) hair keratins present in the hair cortex, hair cuticle and tumors derived therefrom.
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