Product description
Host | guinea pig |
---|---|
Antibody Type | polyclonal |
Immunogen | synthetic human VIP (Peninsula, #7161) |
Purification | undiluted antiserum |
Conjugate | unconjugated |
Formulation | lyophilized; reconstitute in 100 µl dist. water |
Storage | short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles |
No. of experiments | ≤ 4000 (IHC, 50 µl/slide) |
Intended use | Research use only |
Tested species reactivity | cat, guinea pig, human, pig, rabbit, rat |
Applications
Tested applications | Tested dilutions |
---|---|
Immunohistochemistry (IHC) - frozen | 1:1,000 - 1:2,000 |
Immunohistochemistry (IHC) - paraffin | 1:1,000 - 1:2,000 (microwave treatment recommended) |
Background
Details
VIP is localized in nerve fibers of the central and peripheral nervous system, and is probably acting as a neurotransmitter. Smooth muscle relaxation, vasodilation and secretion from exocrine glands are some of the effects of VIP. The Verner-Morrison or Watery Diarrhea Hypokaliemia and Achlorhydria (WDHA) syndrome is a characteristic clinical syndrome associated with overproduction of VIP from endocrine tumors. These VIP-producing tumors are usually neuroblastomas of endocrine tumors in the pancreas.
Absorption with 10-100 µg immunogen per ml diluted antiserum abolishes the staining, while PHI does not.
Positive control: Stefanini-fixed frozen sections of rat intestine
Reference
Filter
whole mount