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anti-Vasoactive Intestinal Peptide rabbit polyclonal, serum

VIP is localized in nerve fibers in the central and peripheral nervous system. It is found in neuroblastomas of endocrine tumors in the pancreas. The antibody is raised against porcine VIP.
Cat. No.: 16099
Quantity:  50 µL

Delivery Time: usually 1-7 working days

€411.00
Excl. 19% Tax, excl. Shipping Cost

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Product description

Host rabbit
Antibody Type polyclonal
Immunogen purified porcine VIP
Purification stabilized antiserum
Conjugate unconjugated
Formulation lyophilized; reconstitute in 50 - 100 μl dist. water (final solution contains 0.09% sodium azide)
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Tested species reactivity cat, fish, human, pig, rat (most mammals)

Applications

Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen 1:1,500 - 1:5,000
Immunohistochemistry (IHC) - paraffin 1:1,500 - 1:5,000 (microwave treatment recommended)

Background

Details

VIP is localized in nerve fibers of the central and peripheral nervous system, and is probably acting as a neurotransmitter. Smooth muscle relaxation, vasodilation and secretion from exocrine glands are some of the effects of VIP. The Verner-Morrison or Watery Diarrhea Hypokaliemia and Achlorhydria (WDHA) syndrome is a characteristic clinical syndrome associated with overproduction of VIP from endocrine tumors. These VIP-producing tumors are usually neuroblastomas of endocrine tumors in the pancreas.
Absorption with 10-100 µg immunogen per ml diluted antiserum abolishes the staining, while PHI, secretin, glucagon, GIP, and CCK do not.

Positive control: Formalin-fixed paraffin sections of cat ileum.

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