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anti-Vesicular Acetylcholine Transporter (human) rabbit polyclonal, lyophilized

The antiserum against the vesicular acetylcholine transporter is a unique immunohistochemical marker for cholinergic nerves. Immunogen: Synthetic peptide from the C-terminus of human VAChT.

Cat. No.: 16095
Quantity:  50 µL
No. of experiments:  ≤ 2400 (IHC, 50 µl/slide)

Delivery Time: usually 1-7 working days

$533.00
Excl. VAT, excl. Shipping Cost

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Specifications

Product description

Host rabbit
Antibody Type polyclonal
Immunogen synthetic peptide from the C-terminus of human VAChT (11 amino acid residues) conjugated to BSA
Purification undiluted antiserum
Conjugate unconjugated
Formulation lyophilized; reconstitute in 100 µl dist. water
Storage short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles
No. of experiments ≤ 2400 (IHC, 50 µl/slide)
Intended use Research use only
Tested species reactivity human

Applications

Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen 1:800 - 1:1,200

Background

Details

The antiserum against the vesicular acetylcholine transporter is a unique immunohistochemical marker for cholinergic nerves, more specific than the commonly used acetylcholinesterase (AchE), since it does not react with postsynaptic neurons, and is more sensitive than choline acetyltransferase (ChAT). The antiserum recognizes VAChT both in the CNS and PNS.
Absorption with 10 – 100 µg immunogen per ml diluted antiserum abolishes staining.

Positive control: Frozen sections of human small intestine (Stefanini fixation).

Reference

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