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Product description
| Host | rabbit |
|---|---|
| Antibody Type | polyclonal |
| Immunogen | synthetic peptide from the C-terminus of rat VAChT conjugated to BSA |
| Purification | undiluted antiserum |
| Conjugate | unconjugated |
| Formulation | lyophilized; reconstitute in 100 µl dist. water |
| Storage | short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles |
| No. of experiments | ≤ 2400 (IHC, 50 µl/slide) |
| Intended use | Research use only |
| Tested species reactivity | mouse, rat (negative with human) |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Immunohistochemistry (IHC) - frozen | 1:800 - 1:1,200 |
| Immunohistochemistry (IHC) - paraffin | 1:800 - 1:1,200 (microwave treatment recommended) |
Background
Details
The antiserum against the vesicular acetylcholine transporter is a unique immunohistochemical marker for cholinergic nerves, more specific than the commonly used acetylcholine esterase (AchE), since it does not react with postsynaptic neurons, and is more sensitive than choline acetyltransferase (ChAT). The antiserum recognizes VAChT both in the CNS and PNS of rat and mouse.
Absorption with 10 – 100 µg immunogen per ml diluted antiserum abolishes staining.
Positive control: Frozen sections of rat small intestine.
Reference
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