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anti-Vesicular Acetylcholine Transporter rabbit polyclonal, serum

The antiserum against the vesicular acetylcholine transporter is a unique immunohistochemical marker for cholinergic nerves, more specific than the commonly used acetylcholinesterase (AchE).

Cat. No.: 16087
Quantity:  50 µL
No. of experiments:  ≤ 2400 (IHC, 50 µl/slide)

Delivery Time: usually 1-7 working days

$499.00
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Specifications

Product description

Host rabbit
Antibody Type polyclonal
Immunogen synthetic peptide from the C-terminus of rat VAChT conjugated to BSA
Purification undiluted antiserum
Conjugate unconjugated
Formulation lyophilized; reconstitute in 100 µl dist. water
Storage short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles
No. of experiments ≤ 2400 (IHC, 50 µl/slide)
Intended use Research use only
Tested species reactivity mouse, rat (negative with human)

Applications

Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen 1:800 - 1:1,200
Immunohistochemistry (IHC) - paraffin 1:800 - 1:1,200 (microwave treatment recommended)

Background

Details

The antiserum against the vesicular acetylcholine transporter is a unique immunohistochemical marker for cholinergic nerves, more specific than the commonly used acetylcholine esterase (AchE), since it does not react with postsynaptic neurons, and is more sensitive than choline acetyltransferase (ChAT). The antiserum recognizes VAChT both in the CNS and PNS of rat and mouse.
Absorption with 10 – 100 µg immunogen per ml diluted antiserum abolishes staining.

Positive control: Frozen sections of rat small intestine.

Reference

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