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Product description
| Host | guinea pig |
|---|---|
| Antibody Type | polyclonal |
| Immunogen | synthetic peptide (CQSYPIGDDEESESD-OH) from the C-terminus of rat VMAT2 conjugated to BSA |
| Purification | undiluted antiserum |
| Conjugate | unconjugated |
| Formulation | lyophilized; reconstitute in 100 µl dist. water |
| Storage | short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles |
| No. of experiments | ≤ 6000 (IHC, 50 µl/slide) |
| Intended use | Research use only |
| Tested species reactivity | human, mouse, pig, rat |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Immunohistochemistry (IHC) - frozen | 1:2,000 - 1:3,000 |
| Western Blot (WB) | assay dependent |
Background
Details
The vesicular monoamine transporter is responsible for the vesicular uptake of monoamines, like dopamine, norepinephrine, epinephrine, serotonin and histamine. The antiserum recognizes monoaminergic neurons of the CNS, the ECL-cells of the stomach, as well as enteric nerve fibers.
Absorption with 10 – 100 µg immunogen per ml diluted antiserum abolishes staining. In Western blot experiments using a crude synaptic vesicle fraction of rat brain, the 70 kD of anti-VMaT2 is recognized (suggested dilution 1:500).
Positive control: Frozen sections of rat small intestine.
Reference
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