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anti-Vimentin guinea pig polyclonal, serum

The anti-Vimentin antibody allows specific detection of 57 kD Vimentin polypeptide in sarcomas, lymphomas and melanomas.
Cat. No.: GP53
Quantity:  100 µL
No. of experiments:  ≤ 100 (IHC, 50 µl/slide); ≤ 40 (WB, 5 ml reaction volume)

Delivery Time: usually 1-7 working days

$306.00
Excl. VAT, excl. Shipping Cost

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Specifications

Product description

Host guinea pig
Antibody Type polyclonal
Immunogen Vimentin purified from calf lens
Purification stabilized antiserum
Conjugate unconjugated
Formulation contains 0.09% sodium azide and 0.5% BSA
Storage short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles
UniprotID P48616
No. of experiments ≤ 100 (IHC, 50 µl/slide); ≤ 40 (WB, 5 ml reaction volume)
Note centrifuge prior to opening
Intended use Research use only
Tested species reactivity bovine, human, mouse

Applications

Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) assay dependent
Immunohistochemistry (IHC) - frozen 1:100
Immunohistochemistry (IHC) - paraffin 1:50 (microwave treatment recommended)
Western Blot (WB) 1:2,000

Background

Details

Specific detection of vimentin (Mr 57,000 polypep-tide).
Tumors specifically detected: Sarcoma (in-cluding myosarcoma), lymphoma, melanoma.

Reference

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