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anti-Vimentin mouse monoclonal, V9, supernatant

The anti-Vimentin antibody decorates mesenchymal cells and mesenchyme-derived tumors (sarcoma, lymphoma, melanoma).
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Cat. No.: 10515
Quantity:  1 mL

Delivery Time: usually 1-7 working days

$262.00
Excl. 19% Tax, excl. Shipping Cost

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Specifications

Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone V9
Immunogen Calf lens
Purification hybridoma cell culture supernatant
Conjugate unconjugated
Formulation contains 0.09% sodium azide
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Note centrifuge prior to opening
Intended use Research use only
Tested species reactivity human, mammals, rat (negative with mouse)

Applications

Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen 1:10
Immunohistochemistry (IHC) - paraffin 1:10 (protease treatment with trypsin and microwave treatment recommended)
Western Blot (WB) assay dependent

Background

Details

The antibody reacts specifically with cells of mesenchmal origin such as fibroblasts, endothelial cells, some smooth muscle cells, chondrocytes, lymphocytes and blood cells. The antibody can be used for differential diagnosis of malignant mesenchymal tumors such as lymphomas, melanomas, sarcomas and carcinomas.
In immunoblotting, the antibody reacts with the 57 kD vimentin protein.
Positive control: Skin fibroblasts.

Reference

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