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human head and neck squamous-cell carcinoma (HNSCC)(courtesy of J.Heß, University Hospital Heidelberg) Zoom

human head and neck squamous-cell carcinoma (HNSCC)(courtesy of J.Heß, University Hospital Heidelberg)

anti-Vimentin mouse monoclonal, VIM 3B4, lyophilized, purified

Excellent marker for mesenchymal cells and mesenchyme-derived tumors (sarcoma, lymphoma, melanoma). The antibody is highly specific for the intermediate filament protein vimentin present in all cells of mesenchymal origin. Most avid mab to vimentin.
application_confirmed highly_published external_validation
Cat. No.: 61013
Quantity:  50 µg
No. of experiments:  ≤ 4000 (IHC, 50 µl/slide); ≤ 100 (WB, 5 ml reaction volume)

Delivery Time: usually 1-7 working days

$295.00
Excl. VAT, excl. Shipping Cost

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Specifications

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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG2a kappa
Clone VIM 3B4
Immunogen Vimentin purified from bovine lens
Purification protein A affinity chromatography
Conjugate unconjugated
Formulation lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09 % sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
Storage short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles
No. of experiments ≤ 4000 (IHC, 50 µl/slide); ≤ 100 (WB, 5 ml reaction volume)
Intended use Research use only
Tested species reactivity amphibia, bovine, chicken, human, monkey (inquire for murine cross-reaction)

Applications

Tested applications Tested dilutions
ELISA assay dependent
Immunocytochemistry (ICC)/ Immunofluorescence (IF) assay dependent
Immunohistochemistry (IHC) - frozen 1:100 - 1:200
Immunohistochemistry (IHC) - paraffin 1:100 - 1:200 (protease treatment and/or microwave treatment recommended)
Western Blot (WB) 1:500

Background

Details

The antibody is highly specific for the intermediate filament protein vimentin which is present in all cells of mesenchymal origin. VIM 3B4 has turned out to be the most avid mab to vimentin.
Polypeptide reacting: Mr 57 000 intermediate filament protein (vimentin) of mesenchymal cells.
Tumors specifically detected: sarcoma (including myosarcoma), lymphoma, melanoma.
The binding region of monoclonal antibody VIM3B4 has been characterized by Bohn et al.(1992). According to these authors, the epitope has been localized on the alpha-helical part of vimentin (rod domain coil 2). Due to an aa substitution at position of aa 353 in murine vimentin (that could explain for the weak cross-reaction of the antibody with murine vimentin) they were able to narrow down the binding region around position 353. These findings were confirmed by truncation mutagenesis experiments using human vimentin (Rogers et al., 1995).

Tested cultured cell lines: fibroblasts (SV-80)

Positive western blot control lysate available:
SV80 western blot control, Cat. No. 64001
SV80 western blot control, large, Cat. No. 64001L

Bohn W, Wiegers W, Beuttenmüller M, Traub P: Species-specific recognition patterns of monoclonal antibodies directed against vimentin. Exp Cell Res 201: 1-7 (1992).
Rogers KR, Eckelt A, Nimmrich V, Janssen K-P, Schliwa M, Herrmann H, Franke WW: Truncation mutagenesis of the non-alpha-helical carboxyterminal tail domain of vimentin reveals contributions to cellular localization but not to filament assembly. Eur J Cell Biol 66: 136-150 (1995).

Reference

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