Alternative Products
Cat. No. | Product Name | Format | Quantity | Price |
---|---|---|---|---|
61013 |
anti-Vimentin mouse monoclonal, VIM 3B4 |
lyophilized, purified | 50 µg |
$295.00
Excl. VAT, excl. Shipping Cost
|
61413 |
anti-Vimentin mouse monoclonal, VIM 3B4 |
FITC Conjugate | 250 µL |
$328.00
Excl. VAT, excl. Shipping Cost
|
61013-WBC |
anti-Vimentin, VIM 3B4 + positive western blot control |
50 µg anti-Vimentin + 50 µg western blot control |
$314.00
|
|
690013 |
anti-Vimentin mouse monoclonal, VIM 3B4 |
liquid, purified | 1 mL (50 µg/mL) |
$425.00
Excl. VAT, excl. Shipping Cost
|
690013S |
anti-Vimentin mouse monoclonal, VIM 3B4 |
liquid, purified, small | 0.2 mL (50 µg/mL) |
$170.00
Excl. VAT, excl. Shipping Cost
|
Product description
Host | mouse |
---|---|
Antibody Type | monoclonal |
Isotype | IgG2a |
Clone | VIM 3B4 |
Immunogen | Vimentin (purified from bovine lens) |
Purification | protein A affinity chromatography |
Conjugate | unconjugated |
Formulation | PBS pH 7.4 with 0.5% BSA and 0.09% sodium azide |
Storage | short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles |
No. of experiments | ≤ 100 (IHC, 50 µl/slide) |
Intended use | Research use only |
Tested species reactivity | amphibia, bovine, chicken, dog, human, monkey (weaker murine cross-reaction) |
Applications
Tested applications | Tested dilutions |
---|---|
ELISA | assay dependent |
Immunocytochemistry (ICC)/ Immunofluorescence (IF) | assay dependent |
Immunohistochemistry (IHC) - frozen | ready-to-use |
Immunohistochemistry (IHC) - paraffin | ready-to-use (protease treatment and/or microwave treatment recommended) |
Western Blot (WB) | assay dependent |
Background
Details
The antibody is highly specific for the intermediate filament protein vimentin which is present in all cells of mesenchymal origin. VIM 3B4 has turned out to be the most avid mab to vimentin.
Polypeptide reacting: Mr 57 000 intermediate filament protein (vimentin) of mesenchymal cells. Tumors specifically detected: sarcoma (including myosarcoma), lymphoma, melanoma. The binding region of monoclonal antibody VIM3B4 has been characterized by Bohn et al.(1992).
According to these authors, the epitope has been localized on the alpha-helical part of vimentin (rod domain coil 2).
Due to an aa substitution at position of aa 353 in murine vimentin (that could explain for the weak cross-reaction of the antibody with murine vimentin) they were able to narrow down the binding region around position 353.
These findings were confirmed by truncation mutagenesis experiments using human vimentin (Rogers et al., 1995).
Reactivity on cultured cell lines: fibroblasts (SV-80)
Rogers, K. R. et al. Truncation mutagenesis of the non-alpha-helical carboxyterminal tail domain of vimentin reveals contributions to cellular localization but not to filament assembly. Eur. J. Cell Biol. 66, 136–50 (1995).
Bohn, W., Wiegers, W., Beuttenmüller, M. & Traub, P. Species-specific recognition patterns of monoclonal antibodies directed against vimentin. Exp. Cell Res. 201, 1–7 (1992).
Reference
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