human skin (courtesy of J.Heß, University Hospital Heidelberg)

anti-Vimentin mouse monoclonal, VIM 3B4, prediluted, purified

Excellent marker for mesenchymal cells and mesenchyme-derived tumors (sarcoma, lymphoma, melanoma). The antibody is highly specific for the intermediate filament protein vimentin present in all cells of mesenchymal origin. Most avid mab to vimentin.

Cat. No.: 65013
Quantity: 5 mL
No. of experiments: ≤ 100 (IHC, 50 µl/slide)

Delivery Time: usually 1-7 working days

$174.00

Excl. VAT, excl. Shipping Cost

Qty:

Publication

Species

Application

Lever, M., Brand-Saberi, B. & Theiss, C. Neurogenesis, gliogenesis and the developing chicken optic tectum: an immunohistochemical and ultrastructural analysis. Brain Struct. Funct. 219, 1009-1024 (2014).

chicken

IHC (frozen)

Heid, H. et al. On the formation of lipid droplets in human adipocytes: the organization of the perilipin-vimentin cortex. PLoS One 9, e90386 (2014).

human

WB
ICC-IF

Moll, R. et al. The cardiac isoform of ?-actin in regenerating and atrophic skeletal muscle, myopathies and rhabdomyomatous tumors: an immunohistochemical study using monoclonal antibodies. Virchows Arch. 449, 175-191 (2006).

human

IHC (paraffin)

BÃ¤r, H. et al. Severe muscle disease-causing desmin mutations interfere with in vitro filament assembly at distinct stages. Proc. Natl. Acad. Sci. U. S. A. 102, 15099-104 (2005).

human

ICC-IF

Koeser, J., Troyanovsky, S. M., Grund, C. & Franke, W. W. De novo formation of desmosomes in cultured cells upon transfection of genes encoding specific desmosomal components. Exp. Cell Res. 285, 114-30 (2003).

human

ICC-IF

Akat, K. et al. Molecular characterization of desmosomes in meningiomas and arachnoidal tissue. Acta Neuropathol. 106, 337-347 (2003).

human

IHC (frozen)

Moll, I., Houdek, P., SchÃ¤fer, S., Nuber, U. & Moll, R. Diversity of desmosomal proteins in regenerating epidermis: immunohistochemical study using a human skin organ culture model. Arch. Dermatol. Res. 291, 437-46 (1999).

human

IHC (frozen)

Demirkesen, C., Hoede, N. & Moll, R. Epithelial markers and differentiation in adnexal neoplasms of the skin: an immunohistochemical study including individual cytokeratins. J. Cutan. Pathol. 22, 518-35 (1995).

human

IHC (paraffin)

Gomi, H. et al. Mice devoid of the glial fibrillary acidic protein develop normally and are susceptible to scrapie prions. Neuron 14, 29-41 (1995).

human

IHC (paraffin)

Herrmann, H., Eckelt, A., Brettel, M., Grund, C. & Franke, W. W. Temperature-sensitive Intermediate Filament Assembly. J. Mol. Biol. 234, 99-113 (1993).

human
xenopus

ICC-IF

Bohn, W., Wiegers, W., BeuttenmÃ¼ller, M. & Traub, P. Species-specific recognition patterns of monoclonal antibodies directed against vimentin. Exp. Cell Res. 201, 1-7 (1992).

human
monkey
rabbit
bovine
chicken
dog
equine

WB
ICC-IF

Rungger-Brandle, E., AchtstÃ¤tter, T. & Franke, W. W. An epithelium-type cytoskeleton in a glial cell: Astrocytes of amphibian optic nerves contain cytokeratin filaments and are connected by desmosomes. J. Cell Biol. 109, 705-716 (1989).

xenopus

IHC (frozen)

Herrmann, H., Fouquet, B. & Franke, W. W. Expression of intermediate filament proteins during development of Xenopus laevis. I. cDNA clones encoding different forms of vimentin. Development 105, 279-98 (1989).

xenopus

WB
IHC (frozen)
ICC-IF

Knapp, A. C. & Franke, W. W. Spontaneous losses of control of cytokeratin gene expression in transformed, non-epithelial human cells occurring at different levels of regulation. Cell 59, 67-79 (1989).

human

WB
ICC-IF

Alternative Products

Cat. No.	Product Name	Format	Quantity	Price
61013	anti-Vimentin mouse monoclonal, VIM 3B4	lyophilized, purified	50 µg	$295.00 Excl. VAT, excl. Shipping Cost
61413	anti-Vimentin mouse monoclonal, VIM 3B4	FITC Conjugate	250 µL	$328.00 Excl. VAT, excl. Shipping Cost
61013-WBC	anti-Vimentin, VIM 3B4 + positive western blot control		50 µg anti-Vimentin + 50 µg western blot control	$314.00
690013	anti-Vimentin mouse monoclonal, VIM 3B4	liquid, purified	1 mL (50 µg/mL)	$425.00 Excl. VAT, excl. Shipping Cost
690013S	anti-Vimentin mouse monoclonal, VIM 3B4	liquid, purified, small	0.2 mL (50 µg/mL)	$170.00 Excl. VAT, excl. Shipping Cost

Product description

Host	mouse
Antibody Type	monoclonal
Isotype	IgG2a
Clone	VIM 3B4
Immunogen	Vimentin (purified from bovine lens)
Purification	protein A affinity chromatography
Conjugate	unconjugated
Formulation	PBS pH 7.4 with 0.5% BSA and 0.09% sodium azide
Storage	short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles
No. of experiments	≤ 100 (IHC, 50 µl/slide)
Intended use	Research use only
Tested species reactivity	amphibia, bovine, chicken, dog, human, monkey (weaker murine cross-reaction)

Applications

Tested applications	Tested dilutions
ELISA	assay dependent
Immunocytochemistry (ICC)/ Immunofluorescence (IF)	assay dependent
Immunohistochemistry (IHC) - frozen	ready-to-use
Immunohistochemistry (IHC) - paraffin	ready-to-use (protease treatment and/or microwave treatment recommended)
Western Blot (WB)	assay dependent

Background

Details

The antibody is highly specific for the intermediate filament protein vimentin which is present in all cells of mesenchymal origin. VIM 3B4 has turned out to be the most avid mab to vimentin.
Polypeptide reacting: Mr 57 000 intermediate filament protein (vimentin) of mesenchymal cells. Tumors specifically detected: sarcoma (including myosarcoma), lymphoma, melanoma. The binding region of monoclonal antibody VIM3B4 has been characterized by Bohn et al.(1992).
According to these authors, the epitope has been localized on the alpha-helical part of vimentin (rod domain coil 2).
Due to an aa substitution at position of aa 353 in murine vimentin (that could explain for the weak cross-reaction of the antibody with murine vimentin) they were able to narrow down the binding region around position 353.
These findings were confirmed by truncation mutagenesis experiments using human vimentin (Rogers et al., 1995).
Reactivity on cultured cell lines: fibroblasts (SV-80)

Rogers, K. R. et al. Truncation mutagenesis of the non-alpha-helical carboxyterminal tail domain of vimentin reveals contributions to cellular localization but not to filament assembly. Eur. J. Cell Biol. 66, 136–50 (1995).
Bohn, W., Wiegers, W., Beuttenmüller, M. & Traub, P. Species-specific recognition patterns of monoclonal antibodies directed against vimentin. Exp. Cell Res. 201, 1–7 (1992).

Reference

Publication

Species

Application

chicken

IHC (frozen)

Heid, H. et al. On the formation of lipid droplets in human adipocytes: the organization of the perilipin-vimentin cortex. PLoS One 9, e90386 (2014).

human

WB
ICC-IF

human

IHC (paraffin)

BÃ¤r, H. et al. Severe muscle disease-causing desmin mutations interfere with in vitro filament assembly at distinct stages. Proc. Natl. Acad. Sci. U. S. A. 102, 15099-104 (2005).

human

ICC-IF

human

ICC-IF

Akat, K. et al. Molecular characterization of desmosomes in meningiomas and arachnoidal tissue. Acta Neuropathol. 106, 337-347 (2003).

human

IHC (frozen)

human

IHC (frozen)

human

IHC (paraffin)

Gomi, H. et al. Mice devoid of the glial fibrillary acidic protein develop normally and are susceptible to scrapie prions. Neuron 14, 29-41 (1995).

human

IHC (paraffin)

Herrmann, H., Eckelt, A., Brettel, M., Grund, C. & Franke, W. W. Temperature-sensitive Intermediate Filament Assembly. J. Mol. Biol. 234, 99-113 (1993).

human
xenopus

ICC-IF

Bohn, W., Wiegers, W., BeuttenmÃ¼ller, M. & Traub, P. Species-specific recognition patterns of monoclonal antibodies directed against vimentin. Exp. Cell Res. 201, 1-7 (1992).

human
monkey
rabbit
bovine
chicken
dog
equine

WB
ICC-IF

xenopus

IHC (frozen)

xenopus

WB
IHC (frozen)
ICC-IF

human

WB
ICC-IF

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anti-Vimentin mouse monoclonal, VIM 3B4, prediluted, purified

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