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Alternative Products
| Cat. No. | Product Name | Format | Quantity | Price |
|---|---|---|---|---|
| 63005 |
Basic Fibroblast Growth Factor, bovine, 50 μg |
50 μg |
$528.00
Excl. VAT, excl. Shipping Cost
|
|
| 63104 |
Basic Fibroblast Growth Factor, bovine, 1 mg |
1 mg |
$2,575.00
Excl. VAT, excl. Shipping Cost
|
Product description
| Purity | > 95% |
|---|---|
| Source | bovine recombinant, produced in E. coli |
Background
Details
Molecular Weight
18 000 (146 aa)
Purification
Heparin affinity chromatography
Biological Activity
≤ 1 ng/ml for half maximum stimulation of cell proliferation with bovine aortic endothelial cells (BAE cells).
Application
Characterized growth factor additive in cell culture media for mesoderm and neuroectoderm-derived cells.
Biological Test System
- Cell proliferation assay with BAE cells
- Rabbit cornea in vivo assay
Reconstitution
Reconstitute in 100 μl (10 μg; 50 μg) dist. H2O, dilute further in PBS as required.
Storage
at 2-8°C (lyoph.)
at -20°C (reconstituted)
Reference
Reference
Abraham JA, Whang JL, Tumolo A, Mergia A, Friedman J, Gospodarowicz D, Fiddes JC: Human basic fibroblast growth factor: Nucelotide sequence and genomic organization. EMBO J 5, 2523-2528 (1986)
Gospodarowicz D, Neufeld G, Schweigerer L: Molecular and biological characterization of fibroblast growth factor, an angiogenic factor which also controls the proliferation and differentiation of mesoderm and neurectoderm-derived cells. Cell Diff 19, 1-176 (1986)
Lobb R, Sasse J, Sullivan R, Shing Y, D'Amore P, Jacobs J, Klagsbrun M: Purification and characterization of heparin-binding endothelial cell growth factors. J Biol Chem 261, 1924-1928 (1986)
Iwane M, Kurokawa T, Sasada R, Seno M, Nakagawa S, Igarashi K: Expression of cDNA-encoding human basic fibroblast growth factor in E. coli. Biochem Biophys Res Comm 146, 470-477 (1987)
Knörzer W, Binder HP, Schneider K, Gruss P, McCarthy JEG, Risau W: Expression of synthetic genes encoding bovine and human basic fibroblast growth factors (bFGFs) in Escherichia coli. Gene 75, 21-30 (1989)
