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Basic Fibroblast Growth Factor, human recombinant, 10 μg

Growth factor additive in cell culture media for mesoderm and neuroectoderm-derived cells. Half maximum stimulation of cell proliferation with bovine aortic endothelial cells (BAE cells) is reached using < 1 ng/ml.
Cat. No.: 63006
Quantity:  10 μg

Delivery Time: usually 1-7 working days

€101.00
Excl. 19% Tax, excl. Shipping Cost

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Product description

Purity > 95%
Source human recombinant, produced in E. coli

Background

Details

Description
Recombinant human basic Fibroblast Growth Factor (hbFGF)

Molecular Weight
18 000

Purification
Heparin affinity chromatography

Biological Activity
≤ 1 ng/ml for half maximum stimulation of cell proliferation with bovine aortic endothelial cells (BAE cells).

Application
Characterized growth factor additive in cell culture media for mesoderm and neuroectoderm-derived cells.

Biological Test System

  • Cell proliferation assay with BAE cells
  • Rabbit cornea in vivo assay

Reconstitution
Reconstitute in 100 μl (10 μg; 50 μg) or 1 ml (1 mg) dist. H2O, dilute further in PBS as required.

Storage
at 2-8°C (lyoph.)
at -20°C (reconstituted)

Reference

Reference

Abraham JA, Whang JL, Tumolo A, Mergia A, Friedman J, Gospodarowicz D, Fiddes JC: Human basic fibroblast growth factor: Nucelotide sequence and genomic organization. EMBO J 5, 2523-2528 (1986)

Gospodarowicz D, Neufeld G, Schweigerer L: Molecular and biological characterization of fibroblast growth factor, an angiogenic factor which also controls the proliferation and differentiation of mesoderm and neurectoderm-derived cells. Cell Diff 19, 1-176 (1986)

Lobb R, Sasse J, Sullivan R, Shing Y, D'Amore P, Jacobs J, Klagsbrun M: Purification and characterization of heparin-binding endothelial cell growth factors. J Biol Chem 261, 1924-1928 (1986)

Iwane M, Kurokawa T, Sasada R, Seno M, Nakagawa S, Igarashi K: Expression of cDNA-encoding human basic fibroblast growth factor in E. coli. Biochem Biophys Res Comm 146, 470-477 (1987)

Knörzer W, Binder HP, Schneider K, Gruss P, McCarthy JEG, Risau W: Expression of synthetic genes encoding bovine and human basic fibroblast growth factors (bFGFs) in Escherichia coli. Gene 75, 21-30 (1989)

Boehnke K, Mirancea N, Pavesio A, Fusenig NE, Boukamp P, Stark H-J: Effects of fibroblasts and microenvironment on epidermal regeneration and tissue function in long-term skin equivalents. Eur J Cell Biol 86, 731-746 (2007)

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