PROGEN - passion for research

close×

Desmin, chicken gizzard, 100 μg

Protein standard for immunoblotting, immunization and immunoassays.
Cat. No.: 62205
Quantity:  100 μg

Delivery Time: usually 1-7 working days

€170.00
Excl. 19% Tax, excl. Shipping Cost

Call us now for more information on our products: +49 6221 8278-0

Any more questions?
PRODUCT REQUEST

Downloads

Product description

Purity > 98%
Source chicken gizzard

Background

Details

Molecular Weight
53,000

Application
Protein standard in 1D and 2D SDS gelelectrophoresis, Immunoassays, Immunization

Isoelectric Point
pI 5.4

Storage
At 2-8°C (lyoph.); at -20°C (reconstituted)

Reconstitution
Reconstitute with 80 μl distilled water (final volume 100 μl)
Reconstitute with 200 μl distilled water (final volume 250 μl)
Final solution: 10 mM sodium phosphate buffer pH 7.5, 6M urea, 2 mM DTT, 1 mM EDTA, 10 mM methylammonium chloride; protein concentration: 1 mg/ml (determined by Bradford method)

Reconstitution to filaments is performed by dissolving in 6 M urea buffer (see above) at concentrations of approx. 0.5 mg/ml. Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl, 2 mM dithiothreitol, 10 mM Tris-HCl, pH 7.4).

For immunization purposes, the solution can be further dialyzed against PBS (phosphate buffered saline, e.g. Dulbecco's PBS).

  • Hatzfeld M and Franke WW (1985). J Cell Biol 101, 1826-1841
  • Hatzfeld M et al. (1987). J Mol Biol 197, 237-255

Reference

Reference

Geisler N and Weber K (1980). Purification of smooth-muscle desmin and a protein-chemical comparison of desmins from chicken gizzard and dog stomach. Eur J Biochem 111, 425

Material safety data sheet

Please complete the form below to request a material safety data sheet

Personal Info

* Required Fields

Product Request

Personal Info

* Required Fields