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Desmin, human recombinant, 100 μg

Human recombinant Desmin for use in immunoblotting and ELISA.
Cat. No.: 62216
Quantity:  100 μg

Delivery Time: usually 1-7 working days

€234.00
Excl. 19% Tax, excl. Shipping Cost

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Product description

Purity > 98%
Source human recombinant, produced in E. coli

Background

Details

Molecular Weight
53,539.6 (calculated from sequence)
55,000 (determined by SDS gelelectrophoresis)

Application
Protein standard in 1D and 2D SDS gelelectrophoresis, Immunoassays, Immunization

Isoelectric Point
pI 5.16 (calculated from sequence)

Reconstitution
Reconstitute with 70 μl distilled water (final volume 100 μl)
Reconstitute with 175 μl distilled water (final volume 250 μl)
Final solution: 30 mM Tris/HCI pH 8, 9.5 M urea, 2 mM DTT, 2 mM EDTA, 10 mM methylammonium chloride; protein concentration: 1 mg/ml

Storage
At 2-8°C (lyoph.); at -20°C (reconstituted)

Reconstitution to filaments:
after desmin is dissolved in 9.5 M urea buffer (see above), protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea and then to low salt condition (50 mM NaCl, 2 mM dithiothreitol, 10 mM Tris-HCl, pH 7.4). For immunization purposes, the solution can be further dialyzed against PBS (phosphate buffered saline, e.g. Dulbecco's PBS).

  • Hatzfeld M. and Franke W.W. (1985). J. Cell Biol. 101, 1826-1841
  • Hatzfeld M. et al. (1987). J. Mol. Biol. 197, 237-255

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