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LNP Anionic Peptide Delivery Kit

Cat. No. PR6102

Prices excl. VAT

Available, delivery time: 1-10 days

€396.00*

Datasheet
Key Features
  • Easy to use
  • For anionic peptides with different lengths (from 3-mer to 28-mer), pI (from 5.9 to 11.9) and % hydrophobic Aa (from 0% to 39%)
  • High stability in diverse biological media
  • Narrow size distribution
  • Robust protection against degradation
  • Suitable for 2D and 3D cell cultures
  • Ideal for proteomics, peptide drug development, and targeted therapy research.
  • Minimal cytotoxicity in vitro and in vivo
  • Available with fluorescence option, facilitating real-time tracking of peptide-loaded nanoparticles in vitro.
Product description
Quantity 1 kit
Storage before reconstitution -20ºC (avoid freeze/thaw cycles)
Storage after reconstitution Up to 60 days at 2-8 ºC
Intended use Research use only
Reconstitution

Formulate in Ethanol 96% per protocol.
Application Nanoparticle reagent kit for anionic peptide delivery
Specificity Peptides
Stability

Do not freeze Anionic Peptide Delivery LNPs.

Do not heat up Anionic Peptide Delivery LNPs.

 
Synonym

LNP
Background

Nanoparticle reagent kit for anionic peptide delivery

The kit ensures efficient interaction with anionic peptides, essential for the development of advanced therapies.

Applications

In vitro

  • Labeling of live cells
  • Intracellular delivery of anionic peptides
  • Therapeutic evaluation
  • Cytotoxicity studies

In vivo

  • Biodistribution evaluation
  • Therapeutic evaluation

About the LNP Anionic Peptide Delivery Kit:

PROGENs Anionic Peptide Delivery LNPs come as a dryed lipid emulsion, easy to formulate by bench-top addition of ethanol. The composition and molar ratios of PROGENs Anionic Peptide Delivery LNPs are optimized for associating and delivering anionic peptides by rendering electrostatic interactions and can be used to efficiently deliver the peptide to specific cell lines and animal models at a recommended concentration of 4 mg/ml. 

PROGENs LNP Anionic Peptide Delivery Kit for peptides carrying a negative net charge plays a pivotal role in fields such as proteomics, peptide drug development, and targeted therapy research. This kit facilitates efficient cellular delivery while preserving peptide integrity and activity, making it an ideal choice for innovative peptide-based treatments. Furthermore, its lipid nanoparticle formulation can be adapted to various administration routes, facilitating smooth transitions from in vitro to in vivo applications through the development of personalized solutions. It has shown efficacy in rodents and zebrafish models across various administration methods.

PROGENs LNP Anionic Peptide Delivery Kit has been validated with peptides that possess different length (from 3-mer to 28-mer), pI (from 5.9 to 11.9) and % hydrophobic Aa (from 0% to 39%) at a recommended concentration of 4 mg/ml. Additionally, PROGEN offers customized solutions with fluorescent reagents, LNPs for cationic peptides and also hydrophobic peptides.

Downloads
File
Category
Size
Filetype
File LNP poster
Category Poster
Size 1.58 MB
Filetype pdf
File LNP Anionic Peptide Delivery Kit Manual
Category Kit manual
Size 263.49 KB
Filetype pdf
Datasheet
FAQs
If necessary, the formulation can be concentrated by using an Amicon Ultra Centrifugal Filter, SpeedVac or Rotavap in mild conditions (avoid overpassing 35 °C or drying out the samples). Samples can be concentrated up to 4-fold its original volume (i.e., to a final volume 250 µL).
Yes, if necessary, the formulation can be filtered using 0.22 µm filters of PES membrane.
Diameter size can be measured by Dynamic Light Scattering (DLS) analysis.
Yes, the formulation can be used in vivo (not for use in humans). For specific recommendations or customized solutions, please contact PROGEN.
This will depend on the specific properties of each molecule. Please refer to the specific protocol of each reagent. For in vivo delivery, formulation can be adapted under request to reach specific requirements attending to the dose and route of administration. For small drugs, it can range from 1% to 15% (55 μg - 0,83 mg) with respect to the total amount of lipids (5,5 mg) per vial. We recommend starting with 1%, and then increasing the concentration gradually. For proteins and peptides, it depends on the selected kit and on the specific molecule, but it is possible to load at least 150 ug of protein/peptide per vial.
We have tested in healthy mice to get an insight, but detailed biodistribution should be done with relevant models and with encapsulated drugs / molecules as this affects the biodistribution. We have conducted s.c. (subcutaneous) administration using one or more imaging modalities as we have optimized labeling (PET, SPECT and fluorescent tracking), i.v. (tail vein injection and retroorbital), i.a. (intra-articular), and i.p. (intraperitoneal).
Once formulated, we recommend using the formulation within 24-48 h, according to the provided protocol.
It is not necessary for initial in vitro testing. There is not a big increase in viscosity and the pH from the formulation is suitable for in vitro testing. It Is also possible to use buffers to formulate, as indicated in the kit protocol.
Filtration through 0,45 μm or 0,22 μm will work to eliminate free precipitated compound to obtain the percentage of free compound and then calculate the percentage of encapsulation through the subtraction of the total and free concentrations, in relation to the total concentration. The filtered fraction that has passed through the filter should be analyzed.
Cargo can enter the cell by either endocytosis or passive membrane fusion.
Yes. Formulations are monodisperse with a diameter size around 100 nm.
1 mL of the Anionic Peptide Delivery Reagent is sufficient to perform 225 delivery experiments in 6-well plates, 1150 experiments in 24-well plates and 6815 experiments in 96-well plates.

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