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Nycodenz®

Freely soluble in water up to 80% (w/v). Suitable for the the isolation of many different cell types from blood and tissue (e.g. monocytes, lymphocytes, granulocytes, platelets, dendritic cells, megakaryotic progenitor cells; liver non-parenchymal cells, islet of Langerhans cells, spermatozoa; plant protoplasts) as well as for the isolation of cell organelles (e.g. nuclei, mitochondria, peroxisomes, lysosomes, endosomal subfractions) and membrane fractions (e.g. Golgi membranes, endoplasmic reticulum, specific plasma membrane domains). Separation of viable from non-viable cells. Also suitable for self-generated gradients. Highly effective for purification of viruses and virus particles. Also suitable for isolation of proteins, plasma lipoproteins and plasmid DNA, fractionation of ribonucleoproteins.
Cat. No.: 1002424
Quantity:  1 x 500 g

Delivery Time: usually 1-7 working days

€438.00
Excl. 19% Tax, excl. Shipping Cost

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Product description

Background

Details

Gradients of Nycodenz® can be generated by:

  • Centrifugation in situ (self-forming gradients).
  • Diffusion. Using Nycodenz®, linear gradients can be simply prepared within 45 minutes.
  • Freezing and thawing.
  • Tilted tube rotation (Gradient Master™).

The density of Nycodenz® in solution can be determined by measuring the refractive index. The density can also be determined spectrophotometrically.

Nycodenz® is a non-particulate medium therefore the distribution of cells in gradients can be determined using a haemocytometer, electronic particle counter or by light scattering measurements using a spectrophotometer.

Nycodenz® does not interfere with the orcinol and diphenylamine reactions for the estimation of nucleic acids nor with the very sensitive dye binding assays for protein and DNA.

Polysaccharides and sugars can be determined in the presence of Nycodenz® using the phenol/H2SO4 assay. Fluorimetric assays of nucleic acids and proteins can also be carried out in the presence of Nycodenz®. Nycodenz® does not interfere with most assays for the marker enzymes of subcellular components, also commercial scintillants are compatible with Nycodenz®.

Nycodenz® can be removed from samples by dialysis, ultrafiltration or gel filtration. Cells, subcellular organelles and other particulate matter can be isolated from Nycodenz® by centrifugation without the risk of contaminating the pellet with Nycodenz®.

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