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NycoPrep™ 1.068

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NycoPrep™ 1.068 is a specially formulated sterile and endotoxin-tested medium devised by Boyum to separate human monocytes.

Cat. No.: 1002351
Quantity:  1 x 100 mL

Delivery Time: usually 1-7 working days

Excl. 19% Tax, excl. Shipping Cost

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Product description

Density 1.068 +/- 0.001 g/mL
Endotoxin <1.0 EU/mL
Osmolality 335 ± 15 mOsm



NycoPrep™ 1.068 is a ready made sterile and endotoxin-tested solution of Nycodenz, 5-(N-2,3-dihydroxypropylacetamido)-2,4,6-triiodo-N,N’-bis(2,3-dihydroxypropyl)isophthalamide, for the in vitro isolation of monocytes from leucocyte-rich plasma.


Nycodenz 13.0 % (w/v)

NaCl 0.58 % (w/v)

Tricine-NaOH, pH 7.4 5 mM

Principle of the separation procedure
Monocytes have a lower average density than lymphocytes, but because the densities of the two cell types overlap, it is difficult to establish a satisfactory separation of high reproducibility, based on density differences alone. The separation of monocytes from lymphocytes can however be greatIy improved by appropriate adjustment of the  osmolality of the gradient medium, while the density is kept constant. By increasing the osmolality the cells expel water, shrink and their density increases (1-3). In this respect lymphocytes are more sensitive than monocytes, thus the density difference between the two types of cell is enhanced. Thus, by using the slightly hyperosmotic solution NycoPrep™ 1.068, pure monocytes can be obtained.

To obtain a good yield of monocytes, use of leucocyte-rich plasma is strongly recommended. Whole blood may be used, but the yields are rather unpredictable and sample handling less easy.


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