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Dot blot with different AAV serotypes and mouse monoclonal anti-AAV9 antibody, clone ADK9 (Courtesy of Regina Heilbronn, Charité Universitätsmedizin Berlin, Mietzsch et al. Hum Gene Ther. 2014 Mar 1; 25(3):212-222) Zoom

Dot blot with different AAV serotypes and mouse monoclonal anti-AAV9 antibody, clone ADK9 (Courtesy of Regina Heilbronn, Charité Universitätsmedizin Berlin, Mietzsch et al. Hum Gene Ther. 2014 Mar 1; 25(3):212-222)

anti-AAV9 (intact particle) mouse monoclonal, ADK9, lyophilized, purified, sample

Useful for analysis of the AAV assembly process. ADK9 specifically reacts with intact adeno-associated virus particles, empty and full capsids. Recognizes a conformational epitope of assembled capsids. Not for use in WB.
Cat. No.: 610162S
Quantity:  10 µg

Delivery Time: usually 1-7 working days

€97.00
Excl. 19% Tax, excl. Shipping Cost

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Specifications

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Product description

Host mouse
Antibody Type monoclonal
Isotype IgA kappa
Clone ADK9
Immunogen recombinant produced adeno-associated virus type 9 (AAV9), carrying GFP
Purification affinity chromatography
Conjugate unconjugated
Formulation lyophilized; reconstitute in 200 µl sterile PBS
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Intended use Research use only
Tested species reactivity AAV9 (published data: no cross-reaction with AAV8 in immunofluorescence microscopy and dot blot )

Applications

Tested applications Tested dilutions
Dot Blot assay dependent (non-denaturing conditions)
ELISA assay dependent
Immunocytochemistry (ICC)/ Immunofluorescence (IF) assay dependent
Immunohistochemistry (IHC) - frozen assay dependent
Immunohistochemistry (IHC) - paraffin assay dependent (microwave treatment recommended)

Background

Details

For characterization of different stages of infection and very useful for the analysis of the AAV assembly process. ADK9 specifically reacts with intact adeno-associated virus particles, empty and full capsids. Recognizes a conformational epitope of assembled capsids.

The antibody cannot be used for immunoblotting.

Reference

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