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Dot blot with different AAV serotypes and mouse monoclonal anti-AAV9 antibody, clone ADK9 (Courtesy of Regina Heilbronn, Charité Universitätsmedizin Berlin, Mietzsch et al. Hum Gene Ther. 2014 Mar 1; 25(3):212-222) Zoom

Dot blot with different AAV serotypes and mouse monoclonal anti-AAV9 antibody, clone ADK9 (Courtesy of Regina Heilbronn, Charité Universitätsmedizin Berlin, Mietzsch et al. Hum Gene Ther. 2014 Mar 1; 25(3):212-222)

anti-AAV9 (intact particle) mouse monoclonal, ADK9, supernatant

Useful for analysis of the AAV assembly process. ADK9 specifically reacts with intact adeno-associated virus particles, empty and full capsids. Recognizes a conformational epitope of assembled capsids. Not for use in WB.
Cat. No.: 651162
Quantity:  5 mL

Delivery Time: usually 1-7 working days

€229.00
Excl. 19% Tax, excl. Shipping Cost

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anti-AAV9 (intact particle) mouse monoclonal, ADK9

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Product description

Host mouse
Antibody Type monoclonal
Isotype IgA kappa
Clone ADK9
Immunogen recombinant produced adeno-associated virus type 9 (AAV9), carrying GFP
Purification hybridoma cell culture supernatant
Conjugate unconjugated
Formulation contains 0.09% sodium azide
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Tested species reactivity AAV9 (published data: no cross-reaction with AAV8 in immunofluorescence microscopy and dot blot )
Intended use Research use only

Applications

Tested applications Tested dilutions
Dot Blot assay dependent (non-denaturing conditions)
ELISA assay dependent
Immunocytochemistry (ICC)/ Immunofluorescence (IF) assay dependent
Immunohistochemistry (IHC) - frozen ready-to-use
Immunohistochemistry (IHC) - paraffin ready-to-use (microwave treatment recommended)

Background

Details

For characterization of different stages of infection and very useful for the analysis of the AAV assembly process. ADK9 specifically reacts with intact adeno-associated virus particles, empty and full capsids. Recognizes a conformational epitope of assembled capsids.

The antibody cannot be used for immunoblotting.

Reference

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