IHC of human colon (© PROGEN in cooperation with Uniklinik Heidelberg, Dr. Jochen Heß)

anti-Desmoglein 1/2 mouse monoclonal, DG 3.10, purified, sample (ready-to-use)

DG 3.10 anti-desmoglein 1/2 antibody recognizes desmoglein 1 + 2 in desmosome-bearing cells; does not react with cells containing plaque-bearing junctions of non-desmosomal type.

Cat. No.: 61002S
Quantity: 600 µl (ready-to-use)

Delivery Time: usually 1-7 working days

$65.00

Excl. 19% Tax, excl. Shipping Cost

Qty:

Publication

Species

Application

Price, A. et al. Mechanical loading of desmosomes depends on the magnitude and orientation of external stress. Nat.Commun. 9, 5284 (2018).

mouse

ICC-IF

Izawa, G., Kobayashi, W., Haraguchi, M., Sudo, A. & Ozawa, M. The ectopic expression of Snail in MDBK cells does not induce epithelial-mesenchymal transition. Int. J. Mol. Med. 36, 166-72 (2015).

bovine

Noritake, K., Aki, T., Funakoshi, T., Unuma, K. & Uemura, K. Direct Exposure to Ethanol Disrupts Junctional Cell-Cell Contact and Hippo-YAP Signaling in HL-1 Murine Atrial Cardiomyocytes. PLoS One (2015).

mouse

Jennemann, R. et al. Loss of ceramide synthase 3 causes lethal skin barrier disruption. Hum. Mol. Genet. 21, 586-608 (2012).

mouse

WB
IHC (paraffin)

Lombardi, R. et al. Nuclear plakoglobin is essential for differentiation of cardiac progenitor cells to adipocytes in arrhythmogenic right ventricular cardiomyopathy. Circ. Res. 109, 1342-53 (2011).

mouse

Co-IP

Rickelt, S., Winter-Simanowski, S., Noffz, E., Kuhn, C. & Franke, W. W. Upregulation of plakophilin-2 and its acquisition to adherens junctions identifies a novel molecular ensemble of cell-cell-attachment characteristic for transformed mesenchymal cells.

human

Pieperhoff, S. & Franke, W. W. The area composita of adhering junctions connecting heart muscle cells of vertebrates - IV: coalescence and amalgamation of desmosomal and adhaerens junction components - late processes in mammalian heart development. Eur. J

mouse

IHC (frozen)

Borrmann, C. M. et al. The area composita of adhering junctions connecting heart muscle cells of vertebrates. II. Colocalizations of desmosomal and fascia adhaerens molecules in the intercalated disk. Eur. J. Cell Biol. 85, 469-485 (2006).

human

IHC (frozen)

Franke, W. W., Borrmann, C. M., Grund, C. & Pieperhoff, S. The area composita of adhering junctions connecting heart muscle cells of vertebrates. I. Molecular definition in intercalated disks of cardiomyocytes by immunoelectron microscopy of desmosomal pr

human

IEM

Bosch, F. X., Andl, C., Abel, U. & Kartenbeck, J. E-cadherin is a selective and strongly dominant prognostic factor in squamous cell carcinoma: a comparison of E-cadherin with desmosomal components. Int. J. Cancer 114, 779-90 (2005).

human

IHC (frozen)

Kartenbeck, J., Haselmann, U. & Gassler, N. Synthesis of junctional proteins in metastasizing colon cancer cells. Eur. J. Cell Biol. 84, 417-430 (2005).

human

IHC (paraffin)
IEM

Peitsch, W. K. et al. Drebrin, an Actin-Binding, Cell-Type Characteristic Protein: Induction and Localization in Epithelial Skin Tumors and Cultured Keratinocytes. J. Invest. Dermatol. 125, 761-774 (2005).

human

IHC (frozen)

Koeser, J., Troyanovsky, S. M., Grund, C. & Franke, W. W. De novo formation of desmosomes in cultured cells upon transfection of genes encoding specific desmosomal components. Exp. Cell Res. 285, 114-30 (2003).

human

WB
ICC-IF

Caldelari, R. et al. A Central Role for the Armadillo Protein Plakoglobin in the Autoimmune Disease Pemphigus Vulgaris. J. Cell Biol. 153, 823-834 (2001).

mouse

Hatzfeld, M., Haffner, C., Schulze, K. & Vinzens, U. The Function of Plakophilin 1 in Desmosome Assembly and Actin Filament Organization. J. Cell Biol. 149, 209-222 (2000)

human

ICC-IF

SchÃ¤fer, S., Stumpp, S. & Franke, W. W. Immunological identification and characterization of the desmosomal cadherin Dsg2 in coupled and uncoupled epithelial cells and in human tissues. Differentiation. 60, 99-108 (1996).

human

Chitaev, N. A. et al. The Binding of Plakoglobin to Desmosomal Cadherins: Patterns of Binding Sites and Topogenic Potential. J. Cell Biol. 113, 359-369 (1996).

human

WB
ICC-IF

Leube, R. E. The topogenic fate of the polytopic transmembrane proteins, synaptophysin and connexin, is determined by their membrane-spanning domains. J. Cell Sci. 108, 883-894 (1995).

human

ICC-IF

Schmelz, M. & Franke, W. W. Complexus adhaerentes, a new group of desmoplakin-containing junctions in endothelial cells: the syndesmos connecting retothelial cells of lymph nodes. Eur. J. Cell Biol. 61, 274-89 (1993).

human
rat
bovine

WB
IHC (frozen)
ICC-IF
IEM

Alternative Products

Cat. No.	Product Name	Format	Quantity	Price
61002	anti-Desmoglein 1/2 mouse monoclonal, DG 3.10	lyophilized, purified	50 µg	$318.00 Excl. 19% Tax, excl. Shipping Cost
70031	anti-Desmosome sample set 2		600 µl each antibody	$184.00

Product description

Host	mouse
Antibody Type	monoclonal
Isotype	IgG1
Clone	DG 3.10
Immunogen	"Band 3" polypeptide of isolated desmosomes from bovine muzzle epidermis
Purification	protein A affinity chromatography
Conjugate	unconjugated
Formulation	PBS pH 7.4 with 0.5% BSA and 0.09% sodium azide
Storage	short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Note	centrifuge prior to opening
Intended use	Research use only
Tested species reactivity	bovine, human, rat

Applications

Tested applications	Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF)	assay dependent
Immunohistochemistry (IHC) - frozen	ready-to-use
Immunohistochemistry (IHC) - paraffin	ready-to-use (microwave treatment recommended)
Western Blot (WB)	assay dependent

Background

Details

DG 3.10 recognizes desmoglein 1 + 2 (desmosome-specific cadherins) in desmosome-bearing cells; does not react with cells containing plaque-bearing junctions of non-desmosomal type. Tumors specifically detected: all epithelium-derived tumors, notably carcinomas, and meningiomas. Polypeptide reacting: Mr 165 000 Desmoglein, "band 3" polypeptide of the desmosomal complex. Epitope recognized has been localized at the repeating unit domain ("RUD") of the cytoplasmic C-terminal part of the polypeptide.

Reactivity on cultured cell lines: A-431, MDBK.

Reference

Publication

Species

Application

Price, A. et al. Mechanical loading of desmosomes depends on the magnitude and orientation of external stress. Nat.Commun. 9, 5284 (2018).

mouse

ICC-IF

Izawa, G., Kobayashi, W., Haraguchi, M., Sudo, A. & Ozawa, M. The ectopic expression of Snail in MDBK cells does not induce epithelial-mesenchymal transition. Int. J. Mol. Med. 36, 166-72 (2015).

bovine

mouse

Jennemann, R. et al. Loss of ceramide synthase 3 causes lethal skin barrier disruption. Hum. Mol. Genet. 21, 586-608 (2012).

mouse

WB
IHC (paraffin)

Lombardi, R. et al. Nuclear plakoglobin is essential for differentiation of cardiac progenitor cells to adipocytes in arrhythmogenic right ventricular cardiomyopathy. Circ. Res. 109, 1342-53 (2011).

mouse

Co-IP

human

mouse

IHC (frozen)

human

IHC (frozen)

human

IEM

human

IHC (frozen)

Kartenbeck, J., Haselmann, U. & Gassler, N. Synthesis of junctional proteins in metastasizing colon cancer cells. Eur. J. Cell Biol. 84, 417-430 (2005).

human

IHC (paraffin)
IEM

human

IHC (frozen)

human

WB
ICC-IF

Caldelari, R. et al. A Central Role for the Armadillo Protein Plakoglobin in the Autoimmune Disease Pemphigus Vulgaris. J. Cell Biol. 153, 823-834 (2001).

mouse

Hatzfeld, M., Haffner, C., Schulze, K. & Vinzens, U. The Function of Plakophilin 1 in Desmosome Assembly and Actin Filament Organization. J. Cell Biol. 149, 209-222 (2000)

human

ICC-IF

human

Chitaev, N. A. et al. The Binding of Plakoglobin to Desmosomal Cadherins: Patterns of Binding Sites and Topogenic Potential. J. Cell Biol. 113, 359-369 (1996).

human

WB
ICC-IF

Leube, R. E. The topogenic fate of the polytopic transmembrane proteins, synaptophysin and connexin, is determined by their membrane-spanning domains. J. Cell Sci. 108, 883-894 (1995).

human

ICC-IF

human
rat
bovine

WB
IHC (frozen)
ICC-IF
IEM

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- By submitting the above request you agree that PROGEN stores and uses the data provided by you in accordance with the terms of use provided in the privacy declaration in order to process your request. You also agree that PROGEN may contact you with regard to your request or for marketing purposes (e.g. Mailchimp-Emailings). You can revoke your permission at any time without giving any reasons by sending an Email to info@progen.com or by writing to PROGEN at Maaßstraße 30, 69123 Heidelberg/Germany.

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anti-Desmoglein 1/2 mouse monoclonal, DG 3.10, purified, sample (ready-to-use)

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