Call +49 6221 8278-0

PROGEN - passion for research

close×

IHC of human colon using anti-Plakophilin 2 (Cat. No. 651101) (courtesy of J.Heß, University Hospital Heidelberg) Zoom

IHC of human colon using anti-Plakophilin 2 (Cat. No. 651101) (courtesy of J.Heß, University Hospital Heidelberg)

anti-Plakophilin 1-3 sample set

The anti-plakophilin 1-3 sample set provides antibodies directed against plakophilin 1, 2 and 3 to evaluate their presence and status in IHC and WB. The set contains enough antibody to perform stainings on 6-12 sections per antibody.

highly_published external_validation
Cat. No.: 70035
Quantity:  600 µl each antibody

 

Cat. No. Product Name Reactivity Application
65160S anti-Plakophilin 1 mouse monoclonal, PP1-5C2, supernatant, sample (ready-to-use) bovine, human, mouse, pig, rat ELISA, IHC, WB
651101S anti-Plakophilin 2 mouse monoclonal, PP2/62 + PP2/86 + PP2/150, supernatant, sample (ready-to-use) bovine, human, rat IHC, WB
651113S anti-Plakophilin 3 mouse monoclonal, PKP3-270.6.2, supernatant, sample (ready-to-use) bovine, human, mouse, rat ICC/IF, IHC, WB

Price as configured: €111.00

Excl. Tax, excl. Shipping Cost

Call us now for more information on our products: +49 6221 8278-0

Any more questions?
PRODUCT REQUEST

Specifications

Product description

Immunogen see individual antibody datasheet for information about specific immunogens
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Number of experiments 6-12 sections per antibody
Note centrifuge prior to opening
Intended use research use only
Tested species reactivity see individual antibody datasheet

Applications

Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen ready-to-use
Immunohistochemistry (IHC) - paraffin ready-to-use (microwave treatment recommended) (anti-Plakophilin 3 antibody not tested)
Western Blot (WB) assay dependent

Background

Details

As one of the stronger cell-to-cell adhesion types desmosomes allow cells to connect their intemediate filaments to those of neighboring cells. Therefore desmosomes can be found especially in cardiac muscle tissue, in bladder tissue and in epithelia, where the meachnical stress is quite high. As members of the cadherin super family, desmogleins and desmocollins thereby mediate adhesion at demosomes. Plakoglobin and the plakophilins on the other hand are members of the armadillo gene family and mediate the connection of the desmosome to desmoplakins that themselves mediate the connection to the cytoskeleton.

Mutations in these proteins or autoimmune reaction against them lead to severe diseases like arrhythmogenic right ventricular cardiomyopathy, Hailey-Hailey disease or striate palmoplantar keratoderma.

Furthermore the tissue-specific expression of desmosomal proteins is critical to tissue homeostasis. Missexpression of single components of desmosomes can lead to hyperproliferation and abnormal differentiation.

The anti-plakophilin 1-3 sample set provides antibodies directed against plakophilin 1, 2 and 3 to evaluate their presence and status in IHC and WB.  The set contains enough antibody to perform stainings on 6-12 sections per antibody.

Set content:
Cat. No. 65160S, anti-Plakophilin 1 mouse monoclonal, PP1-5C2, supernatant, sample (ready-to-use), 600 µl
Cat. No. 651101S, anti-Plakophilin 2 mouse monoclonal, PP2/62 + PP2/86 + PP2/150, supernatant, sample (ready-to-use), 600 µl
Cat. No. 651113S, anti-Plakophilin 3 mouse monoclonal, PKP3-270.6.2, supernatant, sample (ready-to-use), 600 µl

Reference

Reference

PublicationSpeciesApplicationAntibody
Niell, N. et al. The human PKP2/plakophilin-2 gene is induced by Wnt/β-catenin in normal and colon cancer-associated fibroblasts. Int.J.Cancer. 142, 792-804 (2018). human WB 651101
Dubash, A. D. et al. Plakophilin-2 loss promotes TGF-β1/p38 MAPK-dependent fibrotic gene expression in cardiomyocytes. J. Cell Biol. 212, 425–38 (2016). rat WB, ICC-IF 651101
Kant, S., Holthöfer, B., Magin, T. M., Krusche, C. A. & Leube, R. E. Desmoglein 2-Dependent Arrhythmogenic Cardiomyopathy Is Caused by a Loss of Adhesive Function. Circ. Cardiovasc. Genet. 8, 553–63 (2015). mouse IHC (paraffin) 651101
Yang, C. et al. Plakophilin 1-deficient cells upregulate SPOCK1: implications for prostate cancer progression. Tumour Biol. 36, 9567–77 (2015). human IHC (paraffin) 65160
Fischer-Kešo, R. et al. Plakophilins 1 and 3 bind to FXR1 and thereby influence the mRNA stability of desmosomal proteins. Mol. Cell. Biol. 34, 4244–56 (2014). human WB, IP 65160
Rappe, U., Schlechter, T., Aschoff, M., Hotz-Wagenblatt, A. & Hofmann, I. Nuclear ARVCF Protein Binds Splicing Factors and Contributes to the Regulation of Alternative Splicing. J. Biol. Chem. 289, 12421–12434 (2014). human WB 651101
Patel, D. M., Dubash, A. D., Kreitzer, G. & Green, K. J. Disease mutations in desmoplakin inhibit Cx43 membrane targeting mediated by desmoplakin-EB1 interactions. J. Cell Biol. 206, 779–97 (2014). human ICC-IF 651101
Fischer-Kešo, R. et al. Plakophilins 1 and 3 Bind to FXR1 and Thereby Influence the mRNA Stability of Desmosomal Proteins. Mol. Cell. Biol. 34, 4244–4256 (2014). human WB, IP 651101
Rickelt, S. Plakophilin-2: a cell-cell adhesion plaque molecule of selective and fundamental importance in cardiac functions and tumor cell growth. Cell Tissue Res 348, 281–294 (2012). human, mouse WB, IHC (paraffin) 651101
Pieperhoff, S. et al. The plaque protein myozap identified as a novel major component of adhering junctions in endothelia of the blood and the lymph vascular systems. J. Cell. Mol. Med 16, 1709–1719 (2012). mouse WB 651101
Fabritz, L. et al. Load-reducing therapy prevents development of arrhythmogenic right ventricular cardiomyopathy in plakoglobin-deficient mice. J. Am. Coll. Cardiol. 57, 740–750 (2011). mouse IHC 651101
Rickelt, S., Winter-Simanowski, S., Noffz, E., Kuhn, C. & Franke, W. W. Upregulation of plakophilin-2 and its acquisition to adherens junctions identifies a novel molecular ensemble of cell-cell-attachment characteristic for transformed mesenchymal cells. Int. J. cancer 125, 2036–48 (2009). human WB, ICC-IF 651101
Rickelt, S., Winter-Simanowski, S., Noffz, E., Kuhn, C. & Franke, W. W. Upregulation of plakophilin-2 and its acquisition to adherens junctions identifies a novel molecular ensemble of cell-cell-attachment characteristic for transformed mesenchymal cells. Int. J. cancer 125, 2036–48 (2009). human WB 65160
Goossens, S. et al. A unique and specific interaction between  T-catenin and plakophilin-2 in the area composita, the mixed-type junctional structure of cardiac intercalated discs. J. Cell Sci. 120, 2126–2136 (2007). mouse WB, IHC (paraffin), IEM 651101
Pieperhoff, S. & Franke, W. W. The area composita of adhering junctions connecting heart muscle cells of vertebrates – IV: Coalescence and amalgamation of desmosomal and adhaerens junction components – Late processes in mammalian heart development. Eur. J. Cell Biol. 86, 377–391 (2007). mouse IHC (frozen) 651101
Hofmann, I. et al. Identification of the junctional plaque protein plakophilin 3 in cytoplasmic particles containing RNA-binding proteins and the recruitment of plakophilins 1 and 3 to stress granules. Mol. Biol. Cell 17, 1388–98 (2006). human, bovine, xenopus WB, ICC-IF, IEM 65160
Koeser, J., Troyanovsky, S. M., Grund, C. & Franke, W. W. De novo formation of desmosomes in cultured cells upon transfection of genes encoding specific desmosomal components. Exp. Cell Res. 285, 114–30 (2003). human WB, ICC-IF 651101
South, A. P. et al. Lack of plakophilin 1 increases keratinocyte migration and reduces desmosome stability. J. Cell Sci. 116, 3303–3314 (2003). human WB 651101
Akat, K. et al. Molecular characterization of desmosomes in meningiomas and arachnoidal tissue. Acta Neuropathol. 106, 337–347 (2003). human WB, IHC-IF 651101
South, A. P. et al. Lack of plakophilin 1 increases keratinocyte migration and reduces desmosome stability. J. Cell Sci. 116, 3303–3314 (2003). human ICC-IF 65160
Vasioukhin, V., Bowers, E., Bauer, C., Degenstein, L. & Fuchs, E. Desmoplakin is essential in epidermal sheet formation. Nat. Cell Biol. 3, 1076–1085 (2001). human WB, ICC-IF 65160
Hofmann, I. et al. Interaction of plakophilins with desmoplakin and intermediate filament proteins: an in vitro analysis. J. Cell Sci. 113, 2471–83 (2000). human IP, IEM 65160
Mertens, C., Kuhn, C., Moll, R., Schwetlick, I. & Franke, W. W. Desmosomal plakophilin 2 as a differentiation marker in normal and malignant tissues. Differentiation 64, 277–290 (1999). human, bovine IHC (frozen), ICC-IF 651101
Schmidt, A., Langbein, L. & Franke, W. W. Plakophilin 3 - a novel cell type, specific desmosomal plaque. Differentiation 65, 71 (1999). human, bovine, xenopus WB, IHC (frozen), ICC-IF, IEM 65160
Mertens, C., Kulm, C. & Franke, W. W. Plakophilins 2a and 2b: Constitutive Proteins of Dual Location in the Karyoplasm and the Desmosomal Plaque. J. Cell Biol. 135, 1009–1025 (1996). human, bovine IHC (frozen), ICC-IF 651101
Mertens, C., Kulm, C. & Franke, W. W. Plakophilins 2a and 2b: Constitutive Proteins of Dual Location in the Karyoplasm and the Desmosomal Plaque. J. Cell Biol. 135, 1009–1025 (1996). human, bovine IHC (frozen), ICC-IF 65160
Heid, H. W. et al. Cell typespecific desmosomal plaque proteins of the plakoglobin family: plakophilin 1 (band 6 protein). Differentiation 58, 113–131 (1995). human, bovine, xenopus WB, IHC (frozen) 65160

Material safety data sheet

Please complete the form below to request a material safety data sheet

Personal Info

* Required Fields

Product Request

Personal Info

* Required Fields