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IHC of rat tongue (© PROGEN in cooperation with Uniklinik Heidelberg, Dr. Jochen Heß) Zoom

IHC of rat tongue (© PROGEN in cooperation with Uniklinik Heidelberg, Dr. Jochen Heß)

anti-Plakophilin 3 mouse monoclonal, PKP3-270.6.2, supernatant, sample (ready-to-use)

The anti-plakophilin 3 monoclonal antibody stains plakophilin 3, seen in most desmosomes and partly dispersed in the cytoplasm.
Cat. No.: 651113S
Quantity:  600 µL (ready-to-use)

Delivery Time: usually 1-7 working days

€47.00
Excl. 19% Tax, excl. Shipping Cost

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Specifications

Alternative Products

Cat. No. Product Name Format Quantity Price
651113

anti-Plakophilin 3 mouse monoclonal, PKP3-270.6.2

supernatant 5 mL
€196.00
Excl. 19% Tax, excl. Shipping Cost
70032

anti-Desmosome sample set 3

600 µl each antibody
€184.00
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anti-Plakophilin 1-3 sample set

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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone PKP3-270.6.2
Immunogen Recombinant protein (E. coli) of human plakophilin 3
Purification hybridoma cell culture supernatant
Conjugate unconjugated
Formulation hybridoma cell culture supernatant diluted with PBS pH 7.4 containing 0.5% BSA and 0.09% sodium azide
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Note centrifuge prior to opening
Intended use Research use only
Tested species reactivity bovine, human, mouse, rat

Applications

Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) assay dependent
Immunohistochemistry (IHC) - frozen ready-to-use
Immunohistochemistry (IHC) - paraffin ready-to-use (microwave treatment recommended)
Western Blot (WB) assay dependent

Background

Details

Plakophilin 3 is seen in most desmosomes and partly dispersed in cytoplasm under various fixation conditions (FA, MeAc long, MeAc short):

Positive tissues and culture cells:

  • stratified squamous epithelia: palmar, epidermis, scalp skin
  • stratified epithelia: tongue, vagina, esophagus
  • transitional epithelia: bladder
  • non-stratified epithelia: colon, duodenum, pancreas, several glands
  • non-epithelial: reticulum cells of lymph node follicle
  • HaCaT, MCF7, HeLa, HT-29, CaCo 2, Caski, BMGE, MDCK and other

Negative tissues and cell lines:

  • liver, heart, skeletal muscle, endothelia (vessels)
  • PLC, RD, SV80, HL60, Raji, Jok1, Glioma, K562, 3T3L1, A6, PtK2

Reference

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