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WB with anti-AAV VP1/VP2/VP3 antibody (Cat. No. 61058, 1:250) using AAV2 capsids as sample Zoom

WB with anti-AAV VP1/VP2/VP3 antibody (Cat. No. 61058, 1:250) using AAV2 capsids as sample

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Anti-AAV VP1/VP2/VP3 mouse monoclonal, B1, AFDyeTM 647 Conjugate


  • mouse monoclonal

  • AFDyeTM 647 conjugate

  • suitable for western blot analysis

  • reacts with free VP1, VP2, VP3 proteins of AAV1, 2, 3, 5, 6, 7, 8, 9, rh10, DJ

  • isotype: IgG1

Cat. No.: 61058-647
Quantity:  50 µg (100 µl)

Delivery Time: usually 1-7 working days

$498.00
Excl. VAT, excl. Shipping Cost

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Specifications

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Anti-AAV VP1/VP2/VP3 mouse monoclonal, B1

AFDyeTM 488 Conjugate 50 µg (100 µl)
$498.00
Excl. VAT, excl. Shipping Cost

Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone B1
Immunogen AAV2 capsids
Purification protein A affinity chromatography
Conjugate AFDyeTM 647
Formulation liquid; PBS + 0.09% sodium azide
Storage up to 1 month: 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles; store in the dark.
Intended use Research use only
Tested species reactivity AAV1, AAV2, AAV3, AAV5, AAV6, AAV7, AAV8, AAV9, AAVrh10, AAVDJ

Applications

Tested applications Tested dilutions
Dot Blot 1:2,500-1:5,000 (0.1-0.2 µg/ml; denaturing conditions)
Western Blot (WB) 1:2,500-1:5,000 (0.1-0.2 µg/ml; detection limit 5E+09 capsids)

Background

Details

The B1 antibody reacts with free VP1, VP2 and VP3 of adeno-associated virus (AAV) and at a reduced degree with assembled viral particles. VP1 and VP2 are highly enriched in the nucleus, while non-assembled VP3 is evenly distributed in the nucleus and the cytoplasm. Epitope mapping experiments (Wobus et al., 2000) identified aa726 to aa733 (C-terminus; common to all 3 VP proteins) as the specific binding region. The antibody is also useful for characterization of different stages of infection.

The AFDye™ 647 is a bright, far-red-fluorescent dye ideally suited for the 633 nm or 647 nm laser lines (excitation/emission 648/671 nm). It is structurally similar to Alexa Fluor® 647, and can be used with a common Cy5 filter set. A significant advantage to using long wavelength dyes such as Cy5 or AFDye™ 647 dye over other fluorophores is the low autofluorescence of biological specimens in this region of the spectrum.

Wobus, C. E. et al. Monoclonal antibodies against the adeno-associated virus type 2 (AAV-2) capsid: epitope mapping and identification of capsid domains involved in AAV-2-cell interaction and neutralization of AAV-2 infection. J. Virol. 74, 9281–93 (2000).

Alexa Fluor® is a registered trademark of Thermo Fisher Scientific.

Reference

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