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Human pituitary gland

anti-ACTH mouse monoclonal, 2F6, purified

2F6 is specific to synacthen (aa 1-24 of ACTH); does not react with CLIP (aa 17-39 of ACTH).
Cat. No.: 691513
Quantity:  1 mL (100 µg/ml)

Delivery Time: usually 5-10 working days

$425.00
Excl. 19% Tax, excl. Shipping Cost

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Specifications

Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1 kappa
Clone 2F6
Immunogen ACTH synthetic peptide (aa 1-24) conjugated to KLH
Purification affinity chromatography
Conjugate unconjugated
Formulation PBS with 0.02 % sodium azide
Storage store at 2-8°C
Intended use Research use only
Tested species reactivity rat

Applications

Tested applications Tested dilutions
ELISA assay dependent
Flow Cytometry (FACS) 0.5-1.0 µg/million cells in 0.1 ml
Immunocytochemistry (ICC)/ Immunofluorescence (IF) 1:100-1:200 (0.5-1.0 µg/ml)
Immunohistochemistry (IHC) - frozen 1:25-1:50 (2-4 µg/ml)
Immunohistochemistry (IHC) - paraffin 1:25-1:50 (2-4 µg/ml; microwave treatment in 10mM citrate buffer pH 6.0 recommended)

Background

Details

ACTH (same as corticotropin) is a 39 amino acid active peptide produced by the anterior pituitary. 2F6 is specific to synacthen (aa 1-24 of ACTH); does not react with CLIP (aa 17-39 of ACTH). ACTH is also produced by cells of the immune system (T-cells, B-cells, and macrophages) in response to stimuli associated with stress.

Positive control: Pituitary gland, pituitary tumor.

Reference

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