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anti-Actin mouse monoclonal, 2G2, supernatant

Specific for skeletal and non-muscle actin isoforms (Mr 43,000). The antibody also detects nuclear actin isoforms. Epitope location: NVPAMYVAVLDSGVTHNVPIYHAIMRLDLA.
Cat. No.: 651132
Quantity:  5 mL

Delivery Time: usually 1-7 working days

€234.00
Excl. 19% Tax, excl. Shipping Cost

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Specifications

Product description

Host mouse
Antibody Type monoclonal
Isotype IgM
Clone 2G2
Immunogen profilin actin complex from calf thymus
Purification hybridoma cell culture supernatant
Conjugate unconjugated
Formulation contains 0.09% sodium azide
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Intended use Research use only
Tested species reactivity bovine, chicken, human, plant, rabbit

Applications

Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) assay dependent (IF only after methanol fixation)
Immunohistochemistry (IHC) - frozen ready-to-use
Immunohistochemistry (IHC) - paraffin ready-to-use (protease treatment recommended)
Western Blot (WB) assay dependent

Background

Details

Specific for skeletal and non-muscle actin isoforms (Mr 43,000). Epitope location: NVPAMYVAVLDSGVTHNVPIYHAIMRLDLA.

When using antibody 2G2 in immunolocalization studies the presence of nuclear actin could be demonstrated e.g. in cultured cells after fixation with 4% paraformaldehyde. Immunostaining with cytoplasmic actin was only positive after fixation with methanol (Gonsior et al., 1999).

Reactivity on cultured cell lines: PtK2, SR-NRK, NRK-49F, L6 cells, rat fibroblasts, NIH-3T3, mouse myoblast and myotube cells.

Gonsior SM, Platz S, Buchmeier S, Scheer U, Jockusch BM, Hinssen H: Conformational difference between nuclear and cytoplasmic actin as detected by a monoclonal antibody. J Cell Sci 112, 797-809 (1999)

Reference

Reference

PublicationSpeciesApplication
Cruz, J. R. & Moreno Díaz de la Espina, S. Subnuclear compartmentalization and function of actin and nuclear Myosin I in plants. Chromosoma 118, 193–207 (2009). A. cepa WB, ICC-IF
Jockusch, B. M., Schoenenberger, C. A., Stetefeld, J. & Aebi, U. Tracking down the different forms of nuclear actin. Trends Cell Biol. 16, 391–396 (2006). human, rat ICC-IF
Schoenenberger, C. A. et al. Conformation-specific antibodies reveal distinct actin structures in the nucleus and the cytoplasm. J. Struct. Biol. 152, 157–168 (2005). human, rat ICC-IF, dot blot
Bettinger, B. T., Gilbert, D. M. & Amberg, D. C. Actin up in the nucleus. Nat. Rev. Mol. Cell Biol. 5, 410–415 (2004). human, mouse, bovine WB, ICC-IF
Peitsch, W. K. Cell Biological and Biochemical Characterization of Drebrin Complexes in Mesangial Cells and Podocytes of Renal Glomeruli. J. Am. Soc. Nephrol. 14, 1452–1463 (2003). mouse ICC-IF
Zhang, S. et al. Nuclear DNA helicase II/RNA helicase a binds to filamentous actin. J. Biol. Chem. 277, 843–853 (2002). human WB, IEM
Hofmann, W. et al. Cofactor requirements for nuclear export of Rev response element (RRE)- and constitutive transport element (CTE)-containing retroviral RNAs: An unexpected role for actin. J. Cell Biol. 152, 895–910 (2001). xenopus, pleurodeles WB, IEM
Gonsior, S. M. et al. Conformational difference between nuclear and cytoplasmic actin as detected by a monoclonal antibody. J. Cell Sci. 112 ( Pt 6, 797–809 (1999). human WB

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