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WB with anti-Smooth Muscle Actin antibody (Cat. No. 61001, 1:500) on HeLa whole cell lysate; secondary antibody: anti-mouse HRP (Cat. No. 90002, 1:2,500) Zoom

WB with anti-Smooth Muscle Actin antibody (Cat. No. 61001, 1:500) on HeLa whole cell lysate; secondary antibody: anti-mouse HRP (Cat. No. 90002, 1:2,500)

anti-alpha Smooth Muscle Actin mouse monoclonal, 1A4/ ASM-1 + anti-mouse IgG HRP set

The set provides primary anti-alpha Smooth Muscle Actin mouse monoclonal antibody and secondary mouse IgG HRP antibody for western blot. 
The anti-alpha Smooth Muscle Actin antibody represents an excellent marker for myogenic soft tissue tumors and smooth muscle differentiation. Stains leiomyosarkoma, leiomyoma, certain stromal cells surrounding infiltrating ductal carcinoma of breast.

Cat. No.: 71008
Quantity:  50 µg anti-alpha Smooth Muscle Actin + 100 µg anti-mouse IgG HRP

 

Cat. No. Product Name Reactivity Application
61001 anti-alpha-Smooth Muscle Actin mouse monoclonal, 1A4/ ASM-1, lyophilized, purified bovine, chicken, equine, human, mouse, rat ICC/IF, IHC, WB
90002 anti-mouse IgG goat polyclonal, HRP conjugate mouse ELISA, WB

Price as configured: $300.00

Excl. Tax, excl. Shipping Cost

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Specifications

Product description

Immunogen see individual antibody datasheet for information about specific immunogens
Formulation Smooth Muscle Actin antibody: lyophilized; reconstitute in 1 ml dist. water (0.09 % sodium azide, 0.5% BSA in PBS buffer, pH 7.4); anti-mouse IgG HRP antibody: lyophilized, reconstitute in 500 µL dist. water (10 mg/ml BSA in PBS buffer, pH 7.4)
Storage lyophilized at 2-8°C, reconstituted at -20°C (avoid freeze and thaw cycles)
Note centrifuge prior to opening
Intended use research use only
Tested species reactivity see individual antibody datasheet

Applications

Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen anti-alpha Smooth Muscle Actin: dilute at least 1:100
Immunohistochemistry (IHC) - paraffin anti-alpha Smooth Muscle Actin: dilute at least 1:100 (microwave treatment recommended)
Western Blot (WB) anti-alpha Smooth Muscle Actin: 1:500; anti-mouse HRP: 1:2,500 - 1:50,000

Background

Details

anti-Smooth Muscle Actin antibody:
1A4/ ASM-1 represents an excellent marker for myogenic soft tissue tumors and smooth muscle differentiation.
Polypeptide reacting: specific for α-smooth-muscle isoform of actin (Mr 43,000).
Tumors specificallly detected: leiomyosarcoma, leiomyoma, certain stromal cells surrounding infiltrating ductal carcinoma of breast.
Tested cultured cell lines: Stress fibers of smooth muscle-derived cells and some smooth muscle subtype fibroblasts.

anti-mouse IgG goat polyclonal, HRP conjugate:
Whole mouse IgG secondary antibodies are isolated from antisera by immunoaffinity chromatography. The antibody is conjugated to Horseradish peroxidase (HRP) and is commonly used for WB, IHC and ELISA.
Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule mouse IgG. It also reacts with the light chains of other mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody may cross-react with immunoglobulins from other species.

Set content:
Cat. No. 61001, anti-alpha-Smooth Muscle Actin mouse monoclonal, 1A4/ ASM-1, lyophilized, purified
Cat. No. 90002, anti-mouse IgG goat polyclonal, HRP conjugate

Reference

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