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human tonsil

anti-Biotin mouse monoclonal, Hyb-8, purified

Hyb-8 recognizes free biotin and biotinylated proteins (HS biotin, NHS amidocaproylbiotin). The length of the spacer does not affect mAb binding. The antibody also react with biotinylated DNA probes.
Cat. No.: 691698
Quantity:  1 mL (100 µg/ml)
No. of experiments:  ≤ 2000 (IHC, 50 µl/slide); ≤ 40 (WB, 5 ml reaction volume)

Delivery Time: usually 5-10 working days

$425.00
Excl. VAT, excl. Shipping Cost

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Specifications

Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1 kappa
Clone Hyb-8
Immunogen biotinylated sheep immunoglobulin while NHS-biotin was used as a biotinylation agent
Purification affinity chromatography
Conjugate unconjugated
Formulation PBS with 0.02 % sodium azide
Storage store at 2-8°C
No. of experiments ≤ 2000 (IHC, 50 µl/slide); ≤ 40 (WB, 5 ml reaction volume)
Intended use Research use only
Tested species reactivity Biotin

Applications

Tested applications Tested dilutions
ELISA assay dependent
Flow Cytometry (FACS) 0.5-1.0 µg/million cells in 0.1 ml
Immunocytochemistry (ICC)/ Immunofluorescence (IF) 1:100-1:200 (0.5-1.0 µg/ml)
Immunohistochemistry (IHC) - frozen 1:50-1:100 (1-2 µg/ml)
Immunohistochemistry (IHC) - paraffin 1:50-1:100 (1-2 µg/ml)
Western Blot (WB) 1:100-1:200 (0.5-1 µg/ml)

Background

Details

Hyb-8 recognizes free biotin and biotinylated proteins (HS biotin, NHS amidocaproylbiotin). The length of the spacer does not affect mAb binding. The antibody also react with biotinylated DNA probes.

Positive Control: Tissues enriched with biotinylated probes.

Reference

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